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花生籽仁花青素含量近红外模型的构建及应用

Construction and Application of Near Infrared Ray Model for Anthocyanin Content in Peanut Kernel

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花生(Arachis hypogaea L.)籽仁营养丰富,深色种皮富含花青素.选育富含高花青素含量的花生品种需要建立花青素含量的快速检测方法.本研究选用粉色种皮花生品种"宇花91号"(花青素含量182.33 mg/g)与深红色种皮品种"冀花甜1号"(花青素含量270.69 mg/g)杂交构建的F2群体为建模材料,使用Anta-ris Ⅱ型傅里叶变换近红外光谱分析仪对194份样品籽仁进行光谱采集,并测定其种皮花青素含量.利用偏最小二乘法(PLS)构建非损伤性的花青素含量近红外检测模型.该模型的内部验证均方差(RMSECV)为10.3,相关系数R2=0.990 3.选用未参与建模的24份花生材料对该模型进行外部验证,模型预测值和化学测定值的决定系数R2=0.978 1.利用该模型对"宇花91号"与"冀花甜1号"杂交后代群体进行筛选,获得高油酸及高花青素含量种质14份,高糖及高花青素含量种质16份.
Peanut(Arachis hypogaea L.),as an important crop,has rich nutrition in seed kernels and many ac-tive substances in seed coat.It is considered that kernels with dark seed coat are benefit to human health for its high-er anthocyanin content.A simple and easy prediction method on the anthocyanin content is imperative for breeding new varieties with high anthocyanin content.In this study,the F2 population derived from crossing with peanut varie-ty Yuhua 91(pink seed coat with anthocyanin content 182.33 mg/g)and Jihuatian No.1(purple seed coat with an-thocyanin content 270.69 mg/g)was used as the modeling material.The spectrally data of 194 peanut kernels were collected by the Antaris Ⅱ Fourier transform near-infrared spectroscopy,and then the anthocyanin content of peanut seed coat was determined.The partial least squares(PLS)method was used to construct a near-infrared spectrosco-py model for rapid and non-destructive detection on anthocyanin content.The internal validation means square error of cross validation(RMSECV)of the model was 10.3,and the correlation coefficient R2=0.990 3.Twenty-four other peanut materials not involved in the modeling were selected for external verification of the model,and the coeffi-cient of determination of the model prediction value and chemical determination value R2=0.978 1,which indicated that the model could be applied to the rapid detection of anthocyanin content in peanut kernels.The model was used to screen the offsprings from population of Yuhua 91 and Jihuatian No.1,and 14 germplasms with high oleic acid and high anthocyanin content,16 germplasms with high sugar and high anthocyanin content were obtained suc-cessfully.

peanutkernelsanthocyanidin contentNIR modelPLS

李鑫、刘潇洋、于复欣、任建军、周材、周贤涛、邓立苗、唐艳艳、王晶珊、乔利仙

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青岛农业大学农学院,山东省教育厅作物种质创新与应用特色实验室,青岛 266109

香港中文大学(深圳)数据科学学院,深圳 518172

青岛农业大学理学与信息科学学院,青岛 266109

花生 籽仁 花青素含量 近红外模型 偏最小二乘法

青岛市科技局中央引导地方科技发展专项山东省盐碱地农业产业技术体系耐盐碱作物筛选及种质资源开发项目

23-1-3-8-zyydnshSDAIT-29-03

2024

中国粮油学报
中国粮油学会

中国粮油学报

CSTPCD北大核心
影响因子:1.056
ISSN:1003-0174
年,卷(期):2024.39(9)
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