In this study,the extraction of peony seed oil by ultrasonic-assisted aqueous enzymatic method was studied,and the nutritional composition and fatty acid composition of peony seed were determined and analyzed using hazelnut as a reference.With the extraction rate of peony seed oil as the standard of evaluation,the extraction process was optimized by single factor test and response surface methodology.The optimized extraction conditions were ultra-sonic temperature of 49.9 ℃,pH value of 5.3,enzyme dosage of 26 mg/g,and enzymatic hydrolysis time of 4.1 h.Under these conditions,the extraction rate of peony seed oil was 22.48%.The contents of water,protein,fat,ash,total sugar,flavonoids and polyphenols in peony seeds were 7.03%,13.84%,24.60%,2.31%,8.65%,9.81%and 8.78%,respectively.Compared with hazelnuts,peony seeds had lower protein and fat content,but higher fla-vonoid and polyphenol content;the fatty acids in peony seed oil were mainly palmitic acid,stearic acid,oleic acid,linoleic acid and linolenic acid,with the content of 7.02%,1.96%,23.53%,26.92%and 31.38%,respective-ly.The content of linoleic acid and linolenic acid was significantly higher than that of hazelnut oil(11.48%and 0.17%),especially the content of linolenic acid indicating that peony seed oil had higher nutritional value.