The polyphenols in sorghum have positive effects on human health.The technology of ultrasonic-as-sisted ethanol extraction of total polyphenols from sorghum was optimized by single factor and response surface meth-odology.The optimal conditions for ultrasonic extraction of sorghum polyphenols were as follows:ultrasonic time of 58 min,temperature of 57 ℃,ultrasonic power of 450 W,solid-liquid ratio of 1∶17.Under these conditions,the ex-traction rate of sorghum polyphenols was 1.362％.Through the comparison of static adsorption and desorption per-formance of five macroporous resins,HPD-300 resin was determined to be the best resin for separating and purifying sorghum polyphenols,and the optimal purification conditions were 1.33 mg/mL,pH 4,dynamic adsorption speed 2 mL/min,60％ volume fraction of ethanol eluent,and 1.5 mL/min by single factor experiments on sample concen-tration,pH,elution solvent and elution speed.After purification,the purity of lyophilized sorghum polyphenol pow-der was increased by 50.42％,3.3 times of that before purification.Through chemical qualitative analysis,infrared spectroscopy,ultraviolet-visible absorption spectroscopy and liquid chromatography-mass spectrometry,19 phenol-ic acids and 5 tannins were found in sorghum polyphenols.Among them,the content of catechins,protocatechuic acid,phenylalanine,caffeic acid,and gallocatechin was higher.
关键词
高粱/多酚/分离纯化/组分分析
Key words
sorghum/polyphenols/separation and purification/component analysis
维普
万方数据