Optimization of Extraction Process of Polyphenols from Idesia polycarp Meal and in vitro Antioxidant Activity
Idesia polycarpa meal was taken as the research object,single factor experiments were conducted on ethanol concentration,material liquid ratio,ultrasonic temperature,and ultrasonic time.Response surface methodol-ogy was used to optimize the extraction process of polyphenols from idesia polycarpa meal,and the effects of various factors on the extraction rate of polyphenols from idesia polycarpa meal were evaluated.A certain antioxidant activity of polyphenols from idesia polycarpa meal freeze-dried powder was obtained through vacuum freeze-drying.The antioxidant activity was evaluated using DPPH free radical scavenging rate,ABTS free radical scavenging rate,total antioxidant capacity,and reducing capacity measurement methods.The results indicated that the order of influence of various factors was ethanol concentration>ultrasound time>material liquid ratio>ultrasound temperature.The opti-mal extraction process was ethanol concentration of 65％,solid-liquid ratio of 1:60,ultrasound temperature of 63℃,and ultrasound time of 110 minutes.Under these conditions,the extraction rate of polyphenols from idesia poly-carpa meal was 14.16％ in three parallel experiments.The scavenging effect of polyphenols from idesia polycarpa meal freeze-dried powder on DPPH and ABTS free radicals continued to increase with the increase of concentration.At the concentrations of 1.6941 mg/mL and 0.6128 mg/mL,respectively,the scavenging rate was higher than that of the same concentration of vitamin C control.Its reducing ability and total antioxidant capacity increased with in-creasing concentration,with a concentration dependence.Within the concentration range of 0-5 mg/mL,respec-tively,the IC50 is 2.50704 mg/mL and 2.67736 mg/mL.The use of ultrasound assisted solvent extraction of poly-phenols from idesia polycarpa meal avoided resource waste and provided theoretical basis and new ideas for the high-quality utilization of polyphenols from idesia polycarpa meal,having certain reference value.
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