Morphological and molecular identification of Aedes albopictus first reported in Inner Mongolia Autonomous Region,China
Objective To identify whether the Aedes mosquitoes collected in Tumed Left Banner,Hohhot,the Inner Mongolia Autonomous Region,China in 2022 were Ae.albopictus.Methods Six suspected Ae.albopictus mosquitoes were captured in adult mosquito surveillance in August 2022.Their morphological features were characterized.Genomic DNA was extracted from three mosquitoes for amplification and sequencing of the mitochondrial cytochrome c oxidase subunitⅠ(COⅠ)gene and internal transcribed spacer 2(ITS2)gene fragments.The sequencing results were aligned with those in GenBank,and a phylogenetic tree was constructed using the neighbor-joining method.Results The body color and foot characteristics of the collected Aedes mosquitoes were consistent with those of Ae.albopictus.The COⅠand ITS2 alignment results showed the similarities to the sequences of Ae.albopictus were>99.00%and>90.00%,respectively.The phylogenetic tree based on the COⅠ and ITS2 sequences showed that they were all in the same branch as the known Ae.albopictus.Conclusions The Aedes mosquitoes captured in Hohhot in 2022 were morphologically and molecularly identified as Ae.albopictus,and this was the first time that Ae.albopictus was detected in the Inner Mongolia Autonomous Region.
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