Metabolic adaptation associated with diapause in Aedes albopictus induced by low temperature and short sunshine duration
Objective To detect changes in the content of total sugar,glycogen,total cholesterol,triglyceride,sorbitol,and trehalose during diapause in the adults and eggs of Aedes albopictus,and to provide a theoretical basis for the study of diapause physiology of Ae.albopictus.Methods Under laboratory conditions,diapause was induced in Ae.albopictus by short sunshine duration and low temperature,and collected female mosquitoes having laid eggs and eggs in the maintenance phase of diapause to measure the content of biochemical substances(total sugar,glycogen,trehalose,triglyceride,total cholesterol,and sorbitol)using a microplate reader.The experimental data were analyzed by the unpaired t-test with the use of GraphPad 8.0 software.Results In diapausing Ae.albopictus adults,the levels of total sugar,glycogen,trehalose,sorbitol,triglyceride,and total cholesterol were(21.60±2.21),(28.23±0.16),(6.89±0.28),(21.71±3.89),(52.54±4.98)mg/g 和(19.08±1.68)pmol/g,respectively,among which total sugar,glycogen,and sorbitol levels were higher than those of the normal development group(all P＜0.05).The levels of total sugar,glycogen,trehalose,and total cholesterol in diapausing eggs were(103.94±8.06),(8.61±1.54),(1.35±0.07)mg/g,and(13.58±0.28)μmol/g,respectively,all higher than those in the normal development group(all P＜0.01).Conclusions Ae.albopictus adults and eggs have varying increases in biochemical indices under diapause induction conditions,in which the role of total sugar,glycogen,trehalose,and total cholesterol in dispause physiology in mosquitoes deserve further attention.
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