Distribution of knockdown resistance genotypes in Aedes albopictus in Weifang,Shandong Province,China,2022
Objective To detect knockdown resistance mutations in Aedes albopictus in Weifang,Shandong Province,China,so as to provide a scientific basis for Ae.albopictus control.Methods Aedes larvae were collected using the spoon method in the rural residential areas of five counties/cities/districts of Weifang in July 2022,and after emerging as adults in the laboratory,Ae.albopictus were identified.DNA was extracted from each mosquito for PCR-amplifying and sequencing the fragments of the voltage-gated sodium channel gene.Descriptive statistical analysis method was used for the analysis of knockdown resistance mutations in Ae.albopictus.Results A total of 96 mosquitoes were examined,yielding 192 DNA sequences.The length of sequenced fragments was about 400 bp.The sequencing results revealed mutations at three commonly studied loci of the knockdown resistance gene:1016,1532,and 1534.The genotypes of the three loci were predominantly wild-type homozygotes followed by wild/mutant heterozygotes and mutant homozygotes.At locus 1016,there were two alleles,wild-type 1016V(95.31％)and mutant 1016G(4.69％);and there were two genotypes,wild-type homozygous 1016V/N(90.62％)and wild/mutant heterozygous 1016V/G(9.38％).For locus 1532,there were two alleles,which were wild-type 15321(79.17％)and mutant 1532T(20.83％);and there were three genotypes,wild-type homozygous 1532I/I(61.46％),wild/mutant heterozygous 1532I/T(35.42％),and mutant homozygous 1532T/T(3.12％).Three alleles were detected for locus 1534,wild-type 1534F(85.94％),mutant 1534S(1.56％),and mutant 1534C(12.50％);and four genotypes were observed,which were wild-type homozygous 1534F/F(77.08％),wild/mutant heterozygous 1534F/C(17.71％),mutant heterozygous 1534S/C(3.13％),and mutant homozygous 1534C/C(2.08％).For the three loci,10 combinations of the genotypes were observed,and wild-type homozygote combinations were most frequent(36.46％).Conclusion Mutation in the knockdown resistance of Ae.albopictus in Weifang is frequent and complex,and insecticide resistance levels should be closely monitored to guide the scientific use of insecticides.
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