Gene sequence analysis of Hantavirus carried by small mammals in Anhui Province,China,2022
Objective To investigate Hantavirus infection and genotype characteristics in small mammals in Anhui Province,China.Methods From September 2021 to December 2022,small mammal specimens were collected in Anhui Province in accordance with the National Biovector Etiology Surveillance Program(Trial).Hantavirus nucleic acid of small mammal's lung specimens were detected by reverse transcription real-time fluorescence quantitative PCR,and for positive samples,full-length amplification,sequencing,and sequence analysis of the S fragment were conducted.Results A total of 4 075 specimens of 8 species of samll mammals were examined in 16 cities of Anhui Province.The dominant species were Rattus norvegicus(32.15%),Mus musculus(21.87%),R.tanezumi(21.72%),and Apodemus agrarius(20.86%).The positive rate of hantaviral nucleic acids was 1.47%(60/4 075),including Hantaan virus(HNTV)and Seoul virus(SEOV).From the 60 positive samples,19 S sequences were obtained by cross-species PCR amplification.Genetic evolution analysis showed that HNTV was H5 and SEOV was S3 subtypes,respectively.The similarity of the nucleotide sequences was 72.00%to 100%and that of amino acid sequences was 83.00%to 100%,respectively.Conclusions HNTV and SEOV infections were present in small mammals in Anhui Province.It is necessary to strengthen Hantavirus surveillance in small mammals to provide a scientific basis for the prevention and control of hemorrhagic fever with renal syndrome.
Hemorrhagic fever with renal syndromeHantavirusGenotypeS fragment
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