Molecular characteristics of olfaction-related proteins in Haemaphysalis aponommoides
Objective To investigate the molecular basis and mechanism of olfaction of ticks,and to identify the molecular structure of olfaction-related proteins of Haemaphysalis aponommoides and predict their function features.Methods The transcripts of olfaction-related proteins of Ha.aponommoides were acquired through transcriptome sequencing.The secondary and tertiary structures of these proteins were predicted with the use of ESPript 3.0 and Swiss-Model softwares.Based on molecular modeling,the structure features and protein-ligand interaction features of the olfaction-related proteins of Ha.aponommoides were explored.Results A total of 6 olfaction-related transcripts of Ha.aponommoides were acquired,and the corresponding protein sequences were highly homologous to Niemann-Pick C2(NPC2),odorantbinding protein-like protein(OBPL),and microplusin-like protein in Ha.longicornis and Dermacentor silvarum.The OBPL domain consisted of 5 α-helices and 41.83%hydrophobic residues,forming a ligand-binding pocket,and in the NPC2 domain,5 turns and 9 β-sheets comprised a ligand-binding pocket.The ligands of volatile odors were shown overlapped in the center of the pockets with their ligands.Conclusion The OBPL and NPC2 of Ha.longicornis possess a structure enabling odor sensation,suggesting their involvement in olfactory function in ticks.
Haemaphysalis aponommoidesOlfactionNiemann-Pick C2 proteinOdorant binding protein-likeMicroplusin-like protein
NETL
NSTL
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