摘要
目的 对2023年采集自黑龙江省佳木斯市桦南县的蜱标本进行松岭病毒筛查.方法 采用形态学方法对蜱标本进行分类鉴定,反转录实时荧光定量聚合酶链式反应(RT-qPCR)方法对采集的蜱标本进行松岭病毒(Songling virus,SGLV)筛查,并通过Sanger测序法获得阳性病毒基因序列信息.结果 共采集421只蜱标本,包括森林革蜱184只(43.71%),全沟硬蜱 115只(27.32%),日本血蜱 32只(7.60%),嗜群血蜱 43只(10.21%),长角血蜱 7只(1.66%)及未鉴定若蜱40只(9.50%),其中1只雌性嗜群血蜱(编号:2023JMS365)经RT-qPCR检测为SGLV核酸阳性.系统进化分析显示,2023JMS365基因组L、M和S节段与SGLV参考株HLJ1202株的核苷酸一致性分别为96.78%、92.11%和97.59%,氨基酸一致性分别为99.22%、97.50%和93.28%.结论 首次在黑龙江省佳木斯市桦南县的嗜群血蜱中检测到松岭病毒.
Abstract
Objective To detect Songling virus and distribution in ticks collected from Huanan County,Jiamusi,Heilongjiang Province China in 2023.Methods Tick species was identified using morphological methods.The collected tick specimens were examined for Songling virus(SGLV)by using reverse transcription real-time fluorescence quantitative polymerase chain reaction(RT-qPCR).Positive products were sequenced by Sanger sequencing.Results A total of 421 tick specimens were collected,included Dermacentor silvarum(184,43.71%),Ixodes persulcatus(115,27.32%),Haemaphysalis japonica(32,7.60%),H.concinna(43,10.21%),H.longicornis(7,1.66%),and 40 unrecognized nymphs(9.50%).Among them,one female H.concinna(ID:2023JMS365)was tested positive for SGLV nucleic acids by RT-qPCR.The phylogenetic analysis revealed that the nucleotide identencies of the L,M,and S segments of the 2023JMS365 genome were 96.78%,92.11%,and 97.59%respectively with the SGLV reference strain HLJ1202,and the amino acid identencies were 99.22%,97.50%,and 93.28%,respectively.Conclusion SGLV was first detected in H.concinna ticks in Huanan County,Jiamusi City.
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