Detection of Songling virus from ticks in Huanan County,Heilongjiang Province,China
Objective To detect Songling virus and distribution in ticks collected from Huanan County,Jiamusi,Heilongjiang Province China in 2023.Methods Tick species was identified using morphological methods.The collected tick specimens were examined for Songling virus(SGLV)by using reverse transcription real-time fluorescence quantitative polymerase chain reaction(RT-qPCR).Positive products were sequenced by Sanger sequencing.Results A total of 421 tick specimens were collected,included Dermacentor silvarum(184,43.71%),Ixodes persulcatus(115,27.32%),Haemaphysalis japonica(32,7.60%),H.concinna(43,10.21%),H.longicornis(7,1.66%),and 40 unrecognized nymphs(9.50%).Among them,one female H.concinna(ID:2023JMS365)was tested positive for SGLV nucleic acids by RT-qPCR.The phylogenetic analysis revealed that the nucleotide identencies of the L,M,and S segments of the 2023JMS365 genome were 96.78%,92.11%,and 97.59%respectively with the SGLV reference strain HLJ1202,and the amino acid identencies were 99.22%,97.50%,and 93.28%,respectively.Conclusion SGLV was first detected in H.concinna ticks in Huanan County,Jiamusi City.
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