Objective To investigate the pathogens carried by ticks in three northeastern provinces of China and provide reference basis for the prevention and control of tick borne diseases.Methods From 2012 to 2013 and in 2016,tick specimens were collected from 11 counties and cities of northeastern China.Spotted fever group rickettsiae(SFGR)and Bartonella by PCR were tested,Candidatus Neoehrlichia mikurensis,Ehrlichia chaffeensis,and Francisella tularensis by nested PCR,and Borrelia burgdorferi and Babesia microti by quantitative real-time PCR.Results The detection rate was 9.10%for SFGR,4.10%for E.chaffeensis,0.70%for F.tularensis,14.95%for Bor.burgdorferi,and 1.87%for Bab.microti,with a significant difference(χ2=42.808,P<0.001).Bor.burgdorferi and SFGR were most frequently detected,while Candidatus Neoehrlichia mikurensis and Bartonella were not found.There were significant differences between tick species in the positive rates of SFGR(χ2=12.291,P=0.016),Bor.burgdorferi(χ2=23.409,P<0.001),and Bab.microti(χ2=11.592,P=0.028).Conclusions Ticks carry a variety of pathogens in northeast China,in which Bor.burgdorferi and SFGR are more common,and pathogen species differ between tick species,suggesting that these areas should strengthen tick-borne pathogen surveillance to inform the prevention and control of tick-borne diseases.
TickTick-borne pathogenPolymerase chain reactionNortheast China
NETL
NSTL
万方数据
