Detection and genotyping of pathogenic bacteria carried by Rhipicephalus microplus in Ji'an,Jiangxi Province,China
Objective To investigate parasitic ticks and their pathogenic bacteria in Ji'an City,Jiangxi Province,China.Methods A total of 417 parasitic ticks were collected from the body surface of cattle in Yongxin County,Ji'an County,and Yongfeng County of Ji'an City from June 2022 to June 2023.Tick species was identified by morphological examination and 16S rDNA gene analysis.The collected ticks were examined with traditional PCR and nested PCR for six species of tick-borne pathogens,including Borrelia burgdorferi(B.b),B.miyamotoi(B.m),spotted fever group Rickettsia(SFGR),Ehrlichia chaffeensis(EC),Coxiella burnetii(C.b),and Anaplasma phagocytophilum(AP).Positive samples were sequenced.Results All the 417 ticks were identified as Rhipicephalus microplus,and 58 of them tested positive for the pathogens described,with a positive rate of 13.91%.In Yongxin County,B.b,SFGR,and C.b were detected,and the positive rate of B.b was highest(8.99%).In Ji'an County,B.b,EC,and C.b were detected,and the positive rate of C.b was highest(9.21%).In Yongfeng County,B.b,SFGR,C.b,and AP were detected,and the positive rate of SFGR was highest(10.32%).Additionally,co-infections with B.b and other tick-borne pathogens were present in all the three counties.The phylogenetic analysis showed that the parasitic ticks of Ji'an carried three genotypes of B.b(B.yangzensis,B.garinii,and B.barvariensis)and two types of SFGR(R.slovaca and Candidatus R.jingxinensis).Conclusions The species and predominant genotypes of pathogenic bacteria carried by parasitic ticks vary in different counties of Ji'an,and there is a phenomenon of co-infections with multiple bacteria,indicating that pathogenic bacteria carriage in local ticks is complex.Further study and surveillance should be conducted to prevent and control tick-borne diseases in Jiangxi Province.
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