16S rDNA analysis of a pathogen carried by a naturally dead Mustela eversmanii at natural plague foci of Hebei Province
Objective To identify a pathogen isolated from a naturally dead Mustela eversmanni at plague foci of Hebei Province,China,so as to provide a reference for the study of unknown pathogens carried by naturally dead animals at plague foci.Methods Morphologically suspected Yersinia pestis colonies were collected for 16S rDNA gene amplification by polymerase chain reaction and sequencing.Homology alignment and phylogenetic analysis were conducted.Results The 16S rDNA sequence length was 1 432 bp(registration number:OR911995).The sequence had the highest gene homology(99.51%)with NR116898.The phylogenetic tree showed that it belonged to the same evolutionary branch as Hafnia paralvei,and they had the closest genetic relationship.Conclusion H.paralvei was for the first time isolated at natural plague foci in Hebei Province.
16S rDNANatural plague focusMustela eversmaniiHafnia paralveiNaturally dead animalPathogenHebei Province
万方数据
维普
