基于16S rDNA的河北省鼠疫自然疫源地自毙艾鼬携带病原菌研究

16S rDNA analysis of a pathogen carried by a naturally dead Mustela eversmanii at natural plague foci of Hebei Province

刘晓伟 ¹周松 ¹王启盟 ¹白雪薇 ¹王海峰 ¹申小娜 ²张懿晖 ¹闫东 ¹杨顺林 ¹候芝林 ¹杜越聪 ¹栗晓芳 ³杜国义 ¹刘合智¹

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作者信息

1. 河北省鼠疫防治所检验科,河北张家口 075000
2. 中国疾病预防控制中心传染病预防控制所,北京 102206
3. 中国人民解放军陆军第八十一集团军医院,河北张家口 075000
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摘要

目的对分离自河北省鼠疫自然疫源地自毙艾鼬体内的1株病原菌进行鉴定分析,为疫源地内自毙动物携带未知病原菌的研究提供参考依据.方法利用聚合酶链式反应(PCR)对镜检形态疑似鼠疫耶尔森菌的菌落进行16S rDNA基因片段扩增并测序,测序结果经同源性比对后构建系统发育树.结果该菌16S rDNA序列核苷酸长度为1 432 bp(登录号为OR911995).同源性比对结果显示与序列号为NR116898的基因同源性最高(99.51%),系统发育树结果显示,与副蜂房哈夫尼亚菌处于同一进化分支,亲缘关系最近.结论首次自河北省鼠疫自然疫源地分离到副蜂房哈夫尼亚菌.

Abstract

Objective To identify a pathogen isolated from a naturally dead Mustela eversmanni at plague foci of Hebei Province,China,so as to provide a reference for the study of unknown pathogens carried by naturally dead animals at plague foci.Methods Morphologically suspected Yersinia pestis colonies were collected for 16S rDNA gene amplification by polymerase chain reaction and sequencing.Homology alignment and phylogenetic analysis were conducted.Results The 16S rDNA sequence length was 1 432 bp(registration number:OR911995).The sequence had the highest gene homology(99.51%)with NR116898.The phylogenetic tree showed that it belonged to the same evolutionary branch as Hafnia paralvei,and they had the closest genetic relationship.Conclusion H.paralvei was for the first time isolated at natural plague foci in Hebei Province.

关键词

16S/rDNA/鼠疫自然疫源地/艾鼬/副蜂房哈夫尼亚菌/自毙动物/病原菌/河北

Key words

16S rDNA/Natural plague focus/Mustela eversmanii/Hafnia paralvei/Naturally dead animal/Pathogen/Hebei Province

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基金项目

河北省医学科学研究课题计划(20220931)

河北省医学科学研究课题计划(20241280)

出版年

2024

中国媒介生物学及控制杂志

中国疾病预防控制中心

中国媒介生物学及控制杂志

CSTPCDCSCD

影响因子：0.969

ISSN：1003-4692

参考文献量20

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