Screening for a new mosquito repellent formula and its repellent effect against Aedes albopictus in laboratory
Objective To explore the repellent effects of a new mosquito repellent at different concentrations in laboratory,and to compare the indoor repellent effects of formula with additives and other commonly used repellent components on Aedes albopictus,so as to provide an experimental basis for the development of new repellents.Methods The experimental methods were based on the Laboratory Efficacy Test Methods and Criterions of Public Health Insecticides for Pesticide Registration-Part 9:Repellent(GB/T 13917.9-2009).Excel 2007 software was used for data entry and SPSS 22.0 software for statistical analysis.Multivariate repeated measures analysis of variance was used to test the difference in the repellent time of the new repellent and ethanol at various concentrations.The paired samples t-test was used to compare the difference in the repellent time between ethanol solution and the new repellent formula.Random block design and analysis of variance were used to test the differences in the repellent time of the new repellent formula and commonly used repellents against mosquitoes.Results The repellent effect of the new repellent increased with increasing concentration.When the concentration of new repellent was 10%,the repellent time was more than 4 h,and when the concentration was 20%,the repellent time was more than 6 h.The repellent effect of 5%concentration[average repellent time(2.62±0.83)h]was lower than those of 12%,15%,and 20%concentrations[average repellent time(5.13±1.05),(4.94±0.98),and(6.08±1.76)h,all P<0.05].There was no significant difference in the repellent time of new repellent at other concentrations[average repellent time(4.91±1.17)h for 10%concentration,all P>0.05].The repellent effect of formula with 15%ethanol[average repellent time(4.16±1.56)h]was lower than that of the formula with 50%ethanol[average repellent time(5.22±1.53)h,P<0.05].There was no significant difference in the repellent time of formulas with other ethanol concentrations[average repellent time(4.69±1.59)h for 35%ethanol and(4.89±1.77)h for 75%ethanol,all P>0.05].The interaction effect between the new repellent and ethanol was not statistically significant(F=0.849,P=0.601).The repellent time of new repellent formula was increased by 55.25%,16.73%,11.89%,and 9.91%compared with that of 12%ethanol solution(prepared from 15%,35%,50%and 75%ethanol preparations).The repellent effect of new repellent formula[repellent time(6.21±0.97)h]was higher than those of 4.5%IR3535[repellent time(2.89±0.83)h]and 7%N,N-diethyl-meta-toluamide[repellent time(3.54±0.96)h],lower than that of 10%picaridin[repellent time(7.65±0.28)h],and between those of plant essential oil containing p-menthane-3,8-diol[repellent time(5.45±0.75)h]and 15%N,N-diethyl-meta-toluamide[repellent time(6.94±1.41)h].Conclusion The repellent activity of the new repellent has been verified in laboratory,and the new repellent has a good application prospect.
万方数据
维普
