Analysis of genetic characteristics of Coxiella carried by parasitic ticks on goat body surface in Luodian County,Guizhou Province,China
Objective To investigate the basic characteristics of bacteria in the genus Coxiella carried by parasitic ticks on the body surface of goats.Methods Ticks were collected from goats raised in free range in Luodian County,Guizhou Province,China.DNA was extracted from individual ticks and mixed for high-throughput sequencing of bacterial 16S rRNA amplicons.Moreover,individual tick DNA samples were also assayed by PCR with Coxiella-specific primers,which was combined with DNA sequencing to validate the results of high-throughput sequencing.Results A total of 66 ticks were collected in this study,including 63 Haemaphysalis longicornis and 3 Rhipicephalus microplus.A total of 17 reads of Coxiella-like endosymbiont were identified by 16S rRNA high-throughput sequencing.The prevalence rate was 28.78%(19/66)by PCR amplification of the 16S rRNA gene(683 bp)of Coxiella from individual ticks.The phylogenetic analysis based on 16S rRNA gene revealed that the Coxiella-like endosymbionts identified in this study were in the same branch with the Iseral strain(JQ480818)and the Thailand strain(KC170759).Conclusions These findings indicated that the prevalence of Coxiella-like endosymbionts was high in parasitic ticks on the body surface of goats raised in free range in Luodian County.The pathogenicity and the public health significance of the agent require further investigation in the future.
万方数据
维普
