Pathogen surveillance results of Meriones unguiculatus in Inner Mongolia Autonomous Region,China
Objective To detect pathogens in Meriones unguiculatus in the Inner Mongolia Autonomous Region(Inner Mongolia),China,so as to provide a scientific basis for monitoring rodent-borne diseases and vector control.Methods Field rodents were collected in Inner Mongolia in May,July,and September from 2021 to 2023.Nucleic acids were extracted from the spleen of the rodents to detect Bartonella,Dabie bandavirus(DBV),Rickettsia typhi,Anaplasma phagocytophilum,Orientia tsutsugamushi,Francisella tularensis,pathogenic Leptospira,and Orthohantavirus with quantitative PCR(qPCR)/reverse transcription qPCR.The most prevalent pathogen,Bartonella,was further analyzed through rpoB gene sequencing.The positive rates were compared between groups using the Chi-square test.Results A total of 320 samples of M.unguiculatus were tested,of which 40.00%were positive for Bartonella,6.88%were positive for DBV,and<5.00%were positive for any other pathogen.Sequence alignment detected the presence of various Bartonella species,including B.pachyuromydis,B.clarridgeiae,B.rochalimae,B.doshiae,B.grahamii,and B.krasnovii.The positive rate differed significantly in different seasons for Bartonella(highest in autumn,χ2=15.274,P<0.001)and for DBV(highest in spring,χ2=7.247,P=0.025).The highest positive rate(70.59%)of Bartonella was found in Dalai Sumu,New Barag Right Banner,Hulun Buir.Conclusions M.unguiculatus in Inner Mongolia carry various pathogens,with high infection rates of Bartonella and DBV.It is necessary to adjust disease surveillance intensity according to seasons,and strengthen surveillance in high-risk areas such as Dalai Sumu,Hulun Buir.This research provides a reference for risk assessment and early warning of rodent-borne diseases.
万方数据
维普
