An investigation of mosquito species and mosquito-borne virus in some areas of Yunnan Province,China,2023
Objective To investigate mosquito species and mosquito-borne viruses in some areas of Yunnan Province,China in 2023,so as to provide evidence for the prevention and control of mosquito-borne infectious diseases.Methods Mosquitoes were collected in Kaiyuan City(in Honghe Hani and Yi Autonomous Prefecture)and Huaning County and Yuanjiang Hani,Yi,and Dai Autonomous County(in Yuxi City)of Yunnan Province from July to August 2023.After species identification,the mosquitoes were examined for various viruses by quantitative PCR.The target virus gene was amplified by conventional PCR,and the virus sequences were analyzed using bioinformatics software.Results A total of 9 060 mosquitoes belonging to 5 species of 4 genera were collected,among which Culex tritaeniorhynchus was the dominant mosquito species,accounting for 50.30%of the total number of mosquitoes caught,followed by Anopheles sinensis,accounting for 33.77%,Cx.pipiens quinquefasciatus,Armigeres subalbatus and Aedes vexans were less,accounting for 5.96%,2.98%and 2.98%respectively.Ten strains of Japanese encephalitis virus were detected in mosquitoes from Kaiyuan City and Huaning County,with a positive rate of 3.31%.No other viruses were detected.According to the NS1 gene-based phylogenetic analysis and nucleotide and amino acid homology analysis of 8 strains of Japanese encephalitis virus from Kaiyuan City and Huaning County,they all belonged to Japanese encephalitis virus genotype Ⅰ.Conclusions In this investigation,Kaiyuan city and Huaning County were dominated by Cx.tritaeniorhynchus,Yuanjiang County was dominted by An.sinensis.Japanese encephalitis virus was for the first time isolated from mosquitoes in Kaiyuan City and Huaning County,indicating that the two places are epidemic areas of Japanese encephalitis,so surveillance and control should be strengthened.
Mosquito speciesArbovirusJapanese encephalitis virusYunnan Province
NETL
NSTL
万方数据
