摘要
目的 了解贵州省印江土家族苗族自治县(印江县)蜱携带病毒的潜在威胁.方法 在印江县采集牛体表寄生蜱.通过蜱的形态学特征和16S rDNA基因扩增鉴定蜱的种类.病毒宏基因组测序由Illumina平台完成.获得的病毒序列通过质控和组装后与美国国立生物技术信息中心中登录的病毒序列进行比对分析,构建系统发育树,进一步了解其遗传进化特征.结果 2023年7月共采集蜱23只,均为微小扇头蜱.病毒宏基因组测序表明,蜱携带的蜱媒病毒包括白纤病毒科、弹状病毒科和楚病毒科3个病毒科和一些未分类的病毒.本研究获得了白纤病毒科尤库病毒属中1株核苷酸长度为5 008 bp的Lihan病毒序列.遗传进化分析发现贵州省蜱携带的Lihan蜱病毒与2019年在重庆市微小扇头蜱中发现的Lihan蜱病毒分离株序列(ON812189)同源性最高,而与埃及裂谷热病毒(DQ375403)和中国的发热伴血小板减少综合征病毒(JQ733567)(即现大别班达病毒)处于不同遗传进化分支.结论 在贵州省印江县的蜱中发现了3个科的病毒,分别为白纤病毒科、弹状病毒科和楚病毒科.需要更多的研究来进一步了解这些蜱携带病毒的致病性和公共卫生学意义,尤其是本研究中发现的Lihan病毒.
Abstract
Objective To investigate tick-borne virus threats in Yinjiang Tujia and Miao Autonomous County(Yinjiang County),Guizhou Province,China.Methods Ticks were collected from the body surface of cattle in Yinjiang County.Tick species was identified by morphological characterization and 16S rDNA gene amplification.Viral metagenomic sequencing was performed on the Illumina platform.After quality control and assembly,the virus sequences were aligned with those registered in the National Center of Biotechnology Information.A phylogenetic tree was constructed for the genetic evolution characteristics of the viruses.Results A total of 23 ticks were collected in July,2023,all of which were identified as Rhipicephalus microplus.According to metagenomic sequencing,the viruses isolated from the ticks belonged to Phenuiviridae,Rhabdoviridae,and Chuviridae and other unclassified viruses.A 5 008 bp nucleotide sequence of Lihan tick virus was assembled in this study.The sequence had the highest homology to the Lihan virus strain(ON812189)isolated from R.microplus in Chongqing in 2019,belonging to a different genetic branch from those of the Rift Valley fever virus(DQ375403)from Egypt and the Severe fever with thrombocytopenia syndrome virus(JQ733567),namely Dabie bandavirus from China.Conclusions Phenuiviridae,Rhabdoviridae,and Chuviridae viruses were found in ticks in Yinjiang County,Guizhou Province.More studies are needed to understand the pathogenicity and public health significance of these tick-borne viruses,especially the Lihan virus found in this study.
基金项目
国家自然科学基金(82160633)
国家自然科学基金(81760605)
贵州省人民医院科研基金(GPPH-NSFC-2021-17)
贵州省高层次创新型人才基金(黔科合平台人才-GCC[2022]033-1)
NETL
NSTL
万方数据