RNA viruses carried by Rhipicephalus microplus in Yinjiang Tujia and Miao Autonomous County,Guizhou Province,China
Objective To investigate tick-borne virus threats in Yinjiang Tujia and Miao Autonomous County(Yinjiang County),Guizhou Province,China.Methods Ticks were collected from the body surface of cattle in Yinjiang County.Tick species was identified by morphological characterization and 16S rDNA gene amplification.Viral metagenomic sequencing was performed on the Illumina platform.After quality control and assembly,the virus sequences were aligned with those registered in the National Center of Biotechnology Information.A phylogenetic tree was constructed for the genetic evolution characteristics of the viruses.Results A total of 23 ticks were collected in July,2023,all of which were identified as Rhipicephalus microplus.According to metagenomic sequencing,the viruses isolated from the ticks belonged to Phenuiviridae,Rhabdoviridae,and Chuviridae and other unclassified viruses.A 5 008 bp nucleotide sequence of Lihan tick virus was assembled in this study.The sequence had the highest homology to the Lihan virus strain(ON812189)isolated from R.microplus in Chongqing in 2019,belonging to a different genetic branch from those of the Rift Valley fever virus(DQ375403)from Egypt and the Severe fever with thrombocytopenia syndrome virus(JQ733567),namely Dabie bandavirus from China.Conclusions Phenuiviridae,Rhabdoviridae,and Chuviridae viruses were found in ticks in Yinjiang County,Guizhou Province.More studies are needed to understand the pathogenicity and public health significance of these tick-borne viruses,especially the Lihan virus found in this study.
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