Investigation of Rickettsia and Ehrlichia harbored by Haemaphysalis longicornis in Yingkou City,Liaoning Province,China
Objective To investigate the prevalence of Rickettsia and Ehrlichia harbored by Haemaphysalis longicornis in Yingkou City,Liaoning Province,China.Methods A total of 138 free-living ticks were collected using the drag-flag method in Gaizhou county-level City of Yingkou,Liaoning Province.All the ticks were identified as H.longicornis.The samples were pooled for DNA extraction and screened for genera Rickettsia and Ehrlichia using primers targeting their 16S rDNA genes by nested PCR.Subsequently,the gltA,groEL,and 16S rDNA gene sequences were amplified.All the obtained sequences were subjected to genetic and phylogenetic analyses using BLASTN and PhyML 3.0 softwares,respectively.Results Three pooled samples tested positive for Rickettsia,and the species in all samples were identified as Candidatus Rickettsia jingxinensis by sequencing analysis.The 16S rDNA,gltA,and groEL genes showed 100％,100％,and 99.04％ identities to previously reported strains.Moreover,four pooled samples tested positive for Ehrlichia sp.,and 16S rDNA gene sequencing showed 100％ identity to Candidatus E.shimanensis,Ehrlichia sp.Yonaguni 206,and Candidatus E.pampeana.Moreover,the groEL gene showed 100％ identity to Ehrlichia sp.Yonaguni 138,and the gltA gene showed 99.62％ identity (56％ coverage) to uncultured Ehrlichia sp.clone SY130 and 82.85％ identity to E.ewingii.Conclusion The prevalence of Rickettsia and Ehrlichia in ticks from Yingkou of Liaoning Province suggests the potential risk of human infections.
Haemaphysalis longicornisCandidatus Rickettsia jingxinensisEhrlichiaYingkou City
万方数据
维普
