An investigation of rodent-borne pathogens in small mammals in the Chongqing section of the Three Gorges Reservoir area,China,2022-2023
Objective To investigate the prevalence of five rodent-borne pathogens in small mammals in the Chongqing section of the Three Gorges Reservoir area,China,so as to provide scientific guidance for the prevention and control of rodent-borne diseases in the region.Methods From 2022 to 2023,small mammals were captured using the night snap (cage) trapping method in residential areas,cultivated lands,and key industries in the Chongqing section of the Three Gorges Reservoir area.Liver,spleen,and kidney tissues were collected to extract nucleic acids for the detection of Yersinia pestis,Bartonella,Borrelia burgdorferi,Leptospira,and Orientia tsutsugamushi using quantitative real-time PCR (qPCR).Blood samples from the heart were collected to detect Y.pestis F1 antibody,B.burgdorferi IgG antibody,and Leptospira serum antibody using serological methods.Excel 2016 and the SPSS 22.0 softwares were used for data processing and analysis.The pathogen infection rates of small mammals were compared using the Chi-square test or Fisher's exact test.Results A total of 361 small mammals belonging to 10 species were captured,with Rattus tanezumi and R.norvegicus being the dominant species,accounting for 53.46％ and 12.47％,respectively.Detection of liver,spleen,and kidney tissues showed that 9 samples (2.50％) were positive for Leptospira and 17 samples (4.71％) were positive for Bartonella.No samples tested positive for Y.pestis,B.burgdorferi,or O.tsutsugamushi.The overall pathogen infection rates of small mammals were 6.65％.Co-infection of Leptospira and Bartonella was detected in R.tanezumi,and the co-infection rate was 0.55％.Animal serum antibody test results showed that 2 samples (0.80％) were positive for B.burgdorferi IgG antibody,4 samples (1.59％) were positive for Leptospira antibody,and no samples were positive for Y.pestis F1 antibody.Conclusions Small mammals in the Chongqing section of the Three Gorges Reservoir area carry a variety of pathogens including Leptospira,Bartonella,and B.burgdorferi.Surveillance of rodent-borne pathogens carried by small mammals in this region should be enhanced and rodent prevention and control measures should be actively implemented to safeguard the public health of local residents.
Three Gorges Reservoir areaSmall mammalLeptospiraBartonellaBorrelia burgdorferi
万方数据
维普
