Surveillance of main host animals of Leptospira and its antibodies in human population in Baiquan Town,Dinghai District,Zhoushan,Zhejiang Province,China
Objective To investigate the infection of Leptospira in the main host animals and local residents in Baiquan Town,Dinghai District,Zhoushan,Zhejiang Province,China,and assess the potential epidemic risk of leptospirosis,so as to provide a basis for the prevention and control of leptospirosis in Zhoushan islands.Methods Ecological surveillance of host animals was conducted using night clamping and cage trapping methods and kidney samples were collected.The host animals were identified and analyzed for population density and species composition.Quantitative reverse transcription PCR was used to detect the nucleic acids of Leptospira in host kidney and human serum samples.Positive samples were subjected to reverse transcription PCR targeting the 23S rRNA gene for amplification and sequencing to conduct genotyping and homology analysis.ELISA was used to detect IgM and IgG antibodies of Leptospira in human serum.Chi-square test or Fisher's exact test was used to analyze differences between groups.Results In 2023,a total of 234 main hosts (small mammals) were captured in Baiquan Town,with a density of 7.50％ capture rate and a dominant species of Rattus norvegicus (74.07％).The kidney tissues of 59 small mammals were positive for Leptospira nucleic acids,with a positive rate of 25.21％.The kidney tissues of 19 samples were successfully sequenced for 23S rRNA gene,with 13 L.borgpetersenii and 6 L.interrogans.None of the 516 human serum samples were positive for Leptospira nucleic acids,33 (6.40％) samples were positive for IgG,and 12 samples (2.33％) were positive for IgM.Conclusions Baiquan Town is characterized by high density of main host animals,high Leptospira infection rate,and low population immunity rate.In addition,Baiquan Town has a subtropical monsoon climate with heavy rainfall in August,and the potential risk of leptospirosis epidemics is high.
维普
万方数据
