HIF-1α、cyclin-D1在膀胱癌组织中的表达及其临床意义分析
Expressions and clinical significance of HIF-1α and cyclin-D1 in bladder cancer tissues
陈旭 1刘珍 1罗利娟1
作者信息
- 1. 641000 四川省内江市第二人民医院
- 折叠
摘要
目的 分析缺氧诱导因子1α(HIF-1α)、细胞周期蛋白D1(cyclin-D1)在膀胱癌组织中的表达及其临床意义.方法 收集2015年3月-2020年6月该院行手术治疗的146例膀胱癌患者膀胱癌组织和癌旁组织样本,采用免疫组织化学法检测HIF-1α和cyclin-D1表达情况,分析HIF-1α和cyclin-D1表达水平与临床病理的关系,分析膀胱癌患者预后情况,比较不同预后患者癌组织HIF-1α和cyclin-D1表达水平及关系.结果 膀胱癌组织中HIF-1α和cyclin-D1阳性率和表达评分均显著高于癌旁组织(P＜0.05);肿瘤直径≥3cm的HIF-1α和cyclin-D1表达水平显著高于肿瘤直径＜3cm(P＜0.05),临床分期T3～T4的HIF-1α和cyclin-D1表达水平显著高于临床分期T1～T2(P＜0.05),病理分级中,HIF-lα和cyclin-D1表达水平G3＞G2＞G1(P＜0.05),淋巴转移的HIF-1α和cyclin-D1表达水平显著高于未转移(P＜0.05);随访至2023年6月,146例患者出现59例复发(40.41％),87例未复发(59.59％);146例患者存活134例(91.78％),死亡12例(8.22％);复发组HIF-1α和cyclin-D1表达水平显著高于未复发组(P＜0.05),死亡组HIF-1α和cyclin-D1表达水平显著高于存活组(P＜0.05);HIF-1α和cyclin-D1低表达患者无病生存期显著长于高表达患者(log-rank x2=4.237,log-rank x2=6.525,P＜0.05).结论 HIF-1α 和 cyclin-D1 表达水平与膀胱癌病情进展及生存预后相关,可作为临床诊治参考指标.
Abstract
Objective To analyze the expressions and clinical significance of hypoxia-inducible factor 1α(HIF-1α)and cyclin-D1 in bladder cancer tissues.Methods Samples of bladder cancer tissues and adjacent bladder tissues of one hundred and forty-six patients with bladder cancer undergoing surgical treatment in Neijiang Second People's Hospital from March 2015 to June 2020 were collected.The expressions of HIF-1α and cyclin-D1 were detected by immunohistochemistry,and the relationship between expression levels of HIF-1α and cy-clin-D1 and clinicopathology was analyzed.The prognosis of patients with bladder cancer was analyzed and lev-els of HIF-1α and cyclin-D1 in cancer tissues of patients with different prognosis status were compared.Re-sults The positive rates and expression scores of HIF-1α and cyclin-D1 in bladder cancer tissues were signifi-cantly higher than that in adjacent tissues(P＜0.05).The expression levels of HIF-1α and cyclin-D1 in pa-tients with tumor diameter≥3cm were significantly higher than that in patients with tumor diameter＜3cm(P＜0.05).HIF-1α and cyclin-D1 in patients with clinical stage T3-T4 were significantly higher than that in patients with clinical stage T1-T2(P＜0.05).According to pathological grading,HIF-1α and cyclin-D1 were manifested as G3＞G2＞G1(P＜0.05).HIF-1α and cyclin-D1 in patients with lymphatic metastasis were sig-nificantly higher than that without metastasis(P＜0.05).Among 146 patients who were followed up to June 2023,59 cases recurred(40.41％)and 87 cases did not recur(59.59％).134 cases(91.78％)survived and 12 cases(8.22％)died among 146 patients.HIF-1α and cyclin-D1 in recurrence group were significantly higher than that in non-recurrence group(P＜0.05),and the levels in death group were significantly higher than that in survival group(P＜0.05).The disease-free survival time of patients with low HIF-1α and cyclin-D1 expressions was significantly longer than that in patients with high expressions(log-rank x2=4.237,log-rank x2=6.525,P＜0.05).Conclusion HIF-1α and cyclin-D1 are correlated with disease progression and survival prognosis of bladder cancer,and can be used as reference indicators for clinical diagnosis and treatment.
关键词
缺氧诱导因子1α/细胞周期蛋白D1/膀胱癌/免疫组织化学Key words
Hypoxia-inducible factor 1α/Cyclin-D1/Bladder cancer/Immunohistochemistry引用本文复制引用
出版年
2024
NETL
NSTL
万方数据