TLR4/ERK1/2信号通路在不明原因自然流产蜕膜组织中的作用研究
Role of TLR4/ERK1/2 signaling pathway in decidual tissue of unexplained spontaneous abortion
李娜 1栾兆进 1杨美霞 1宫晓玲 2赵紫薇 1宋芳1
作者信息
- 1. 包头医学院组织学与胚胎学教研室,包头 014000
- 2. 包钢第三职工医院,包头 014000
- 折叠
摘要
目的:探讨Toll样受体4(TLR4)和细胞外信号调节蛋白激酶1/2(ERK1/2)在不明原因自然流产患者蜕膜组织中的表达情况及二者的相关性.方法:分别采用免疫组织化学和Western blot技术检测32例不明原因自然流产患者(流产组)和32例正常妊娠者(对照组)蜕膜组织TLR4、ERK1/2和p-ERK1/2的表达差异及表达水平;采用Pearson等级相关性分析流产组TLR4与p-ERK1/2之间的相关性.结果:在免疫组织化学实验中,蜕膜细胞的胞质是TLR4、ERK1/2和p-ERK1/2的表达定位点,且3种蛋白表达有所差异,在流产组TLR4和p-ERK1/2的表达均明显高于对照组(P<0.01),ERK1/2的表达在两组中相较差异无统计学意义(P>0.05),流产组TLR4的蛋白水平高于对照组(P<0.05),p-ERK1/2的蛋白水平明显高于对照组(P<0.01),而ERK1/2的蛋白水平与对照组相比差异无统计学意义(P>0.05);在流产组TLR4与p-ERK1/2的表达呈正相关(r=0.890,P<0.01).结论:TLR4/ERK1/2信号通路的异常激活可能是不明原因自然流产发生机制之一.
Abstract
Objective:To investigate the expression of Toll-like receptor 4(TLR4)and extracellular signal-regulated protein kinase 1/2(ERK1/2)in decidua tissue of patients with unexplained spontaneous abortion and their correlation.Methods:The expres-sions of TLR4,ERK1/2 and p-ERK1/2 in decidua tissues of 32 patients with unexplained spontaneous abortion(abortion group)and 32 normal pregnancy(control group)were detected by immunohistochemistry and Western blot,respectively.The correlation between TLR4 and p-ERK1/2 in abortion group were analyzed by Pearson hierarchical correlation analysis.Results:In immunohistochemical experiments,the cytoplasm of decidua cells in the two groups is the expression locus of TLR4,ERK1/2 and p-ERK1/2,the expression of the three proteins were different,and the expressions of TLR4 and p-ERK1/2 in abortion group were significantly higher than that in control group(P<0.01).There was no significant difference in ERK1/2 expression between abortion group and control group(P>0.05);In decidua tissue samples of abortion group,the protein level of TLR4 was higher than that of control group(P<0.05);the pro-tein level of p-ERK1/2 was significantly higher than that of control group(P<0.01),and the protein level of ERK1/2 in decidua tissue of abortion group was not statistically different from that of control group(P>0.05).TLR4 was positively correlated with p-ERK1/2 expression in abortion group(r=0.890,P<0.01).Conclusion:Abnormal activation of TLR4/ERK1/2 signaling pathway may be one of the mechanisms of unexplained spontaneous abortion.
关键词
TLR4/ERK1/2/p-ERK1/2/自然流产/蜕膜Key words
TLR4/ERK1/2/p-ERK1/2/Spontaneous abortion/Decidual引用本文复制引用
基金项目
内蒙古自治区自然科学基金(2019MS08191)
内蒙古自治区高等学校科研项目(NJZY20175)
包头医学院科学研究基金(BYJJ-QM-2018005)
出版年
2024
国家科技期刊平台
NSTL
万方数据