槲皮素对脓毒症大鼠急性肺损伤及炎症和氧化应激的影响
Effects of quercetin on acute lung injury,inflammation and oxidative stress in sepsis rats
袁国娜 1贺晓云 2李志芳 2蒲柯 3樊波4
作者信息
- 1. 达州中医药职业学院康复系,达州 635000
- 2. 达州中医药职业学院中医系,达州 635000
- 3. 川北医学院附属医院消化科,南充 637000
- 4. 应急总医院医务处,北京 100028
- 折叠
摘要
目的:探究槲皮素(QUE)对脓毒症急性肺损伤(ALI)大鼠的保护作用.方法:60只SD雄性大鼠随机分为6组(n=10):假手术组(Sham)、模型组(CLP)、QUE 25 mg/kg组、QUE 50 mg/kg组、QUE 100 mg/kg组及阳性药物地塞米松(DEX)组.各组连续处理7 d,计算各组大鼠生存率;HE染色和肺湿干重比(W/D)评估肺损伤严重程度;TUNEL染色检测肺组织凋亡;试剂盒检测炎症因子、SOD和MDA水平;Western blot检测大鼠肺组织中凋亡相关蛋白表达及PTEN、β连环蛋白(β-catenin)、蛋白激酶B(AKT)和糖原合酶激酶-3β(GSK-3β)的磷酸化水平.结果:与CLP组比较,QUE 50 mg/kg 和 100 mg/kg处理后,大鼠生存率显著升高(P<0.05),肺组织炎症细胞浸润程度减轻,肺损伤评分和W/D降低(P<0.05),肺组织细胞凋亡率和凋亡相关蛋白表达显著降低(P<0.05),炎症因子水平下降,抗氧化能力增强(P<0.05),PTEN和β-catenin磷酸化水平降低,AKT 和GSK-3β磷酸化水平升高(P<0.05).结论:QUE通过抑制凋亡、降低炎症反应和抗氧化保护大鼠脓毒症ALI,其机制可能与PTEN/β-catenin和AKT/GSK-3β通路相关.
Abstract
Objective:To investigate the protective effect of quercetin(QUE)on acute lung injury(ALI)rats with sepsis.Methods:Sixty male SD rats were randomly divided into 6 groups(n=10):Sham operation group(Sham),model group(CLP),QUE 25 mg/kg group,QUE 50 mg/kg group,QUE 100 mg/kg group and positive drug dexamethasone(DEX)group.Rats in each group were continuously treated for 7 days,and the survival rate was calculated;HE staining and lung wet-to-dry weight ratio(W/D)were used to evaluate the severity of lung injury;TUNEL staining was used to detect lung tissue apoptosis;levels of inflammatory factors,SOD and MDA were detected by the kits;Western blot was used to detect expressions of apoptosis-related proteins and phosphoryla-tion levels of PTEN,β-catenin,protein kinase B(AKT)and glycogen synthase kinase-3β(GSK-3β)in rat lung tissue.Results:Com-pared with CLP group,after QUE 50 mg/kg and 100 mg/kg treatment,survival rate of rats was significantly increased(P<0.05),the degree of inflammatory cell infiltration in lung tissue was reduced,lung injury score and W/D were reduced(P<0.05),apoptosis rate of lung tissue cells and expressions of apoptosis-related proteins were significantly decreased(P<0.05),levels of inflammatory factors were decreased(P<0.05),while the antioxidant capacity was enhanced(P<0.05),phosphorylation levels of PTEN and β-catenin were decreased,while phosphorylation levels of AKT and GSK-3β were increased(P<0.05).Conclusion:QUE protects rats from ALI with sepsis by inhibiting apoptosis,reducing inflammation and antioxidance,which mechanism may be related to PTEN/β-catenin and AKT/GSK-3β pathways.
关键词
槲皮素/急性肺损伤/凋亡/炎症因子/PTEN/β-cateninKey words
Quercetin/Acute lung injury/Apoptosis/Inflammatory factors/PTEN/β-catenin引用本文复制引用
基金项目
四川省科技厅应用基础研究项目(2020YJ0433)
出版年
2024
国家科技期刊平台
NSTL
万方数据