单细胞测序分析病毒性肺炎患者T细胞受体基因差异
Single cell sequencing analyzes T cell receptor gene differences of patients with viral pneumonia
马恩泽 1宁可 1朱晓2
作者信息
- 1. 哈尔滨师范大学计算机科学与信息工程学院,哈尔滨 150025
- 2. 烟台大学计算机与控制工程学院,烟台 264005
- 折叠
摘要
目的:采用单细胞测序分析病毒性肺炎患者外周血T细胞受体(TCR)基因差异,探讨可能发病机制.方法:利用NCBI数据库获得数据,设置病例组和对照组进行实验.使用RStudio软件R语言分析单细胞测序数据,绘制表格和图片,得出结论.结果:病例组和对照组TCR有显著差异.对TCR α链和β链V、J基因片段使用频率进行分析,其中病例组TRAV1-2、TRAV29/DV5、TRAJ33、TRAJ48、TRBV20-1、TRBV2、TRBJ2-3、TRBJ1-1等基因使用频率明显高于对照组(P<0.05).进一步V-J基因组合分析显示,病例组α链V-J对使用频率最高的是TRAV1-2-J33,β链V-J使用频率最高的是TRBV20-1-J2-1,αβ双链V-J使用频率最高的是TRAV30-J24-TRBV3-1-J2-7.结论:病毒性肺炎患者外周血TCR基因发生了明显改变,可能与病毒免疫发病机制有关.
Abstract
Objective:To analyze differences in peripheral blood T-cell receptor(TCR)genes in patients with viral pneumo-nia by single cell sequencing,and to explore its possible pathogenesis.Methods:Data in NCBI database was obtained,and case and control groups were set up for experiments.Single cell sequencing data was analyzed of by R language in RStudio software,tables and images were plotted,and finally conclusions were drawn.Results:There was a significant difference in TCRs between case and healthy control groups.Frequency of use of V and J gene fragments of TCR α and β chains were analyzed,in which frequency of use of TRAV1-2,TRAV29/DV5,TRAJ33,TRAJ48,TRBV20-1,TRBV2,TRBJ2-3 and TRBJ1-1 genes were significantly higher in case group than in control group(P<0.05).Further V-J gene combination analysis showed that the most frequent use of α-chain V-J pairs in case group was TRAV1-2-J33,most frequent use of β-chain V-J pairs was TRBV20-1-J2-1,most frequent use of αβ double-chain V-J pairs was TRAV30-J24-TRBV3-1-J2-7.Conclusion:Peripheral blood TCR genes are significantly altered in patients with viral pneu-monia,which may be related to immunopathogenesis of virus.
关键词
单细胞测序/病毒性肺炎/T细胞受体/基因Key words
Single cell sequencing/Viral pneumonia/TCR/Gene引用本文复制引用
基金项目
国家自然科学基金青年科学基金(61902094)
黑龙江省自然科学基金(QC2018082)
出版年
2024
国家科技期刊平台
NSTL
万方数据