首页|中药经方二妙散对Gnαq表达的影响及改善CIA模型大鼠炎症反应研究

中药经方二妙散对Gnαq表达的影响及改善CIA模型大鼠炎症反应研究

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
目的:探讨经方二妙散(EMS)对胶原诱导型关节炎(CIA)大鼠脾脏和关节中免疫调控基因 Gnαq 表达的影响.方法:建立CIA大鼠模型,并随机分为模型组(CIA组)、1.5、3.0和4.5 g/kg二妙散组(L-EMS、M-EMS和H-EMS组)、阳性对照组(MTX,氨甲蝶呤),各组给予相应药物进行治疗,同时设立健康对照组(Control组)与模型组给予等量生理盐水灌胃.ELISA技术检测各组炎症因子IL-1β、IL-6和TNF-α的表达,使用qRT-PCR和Western blot分析Gnαq mRNA和蛋白的表达,免疫组化技术对脾脏和关节中的Gnαq进行定位.结果:CIA组大鼠关节出现明显组织受损、组织增生与炎症浸润等特征改变;MTX组和EMS组关节组织破坏及增生减轻;CIA组IL-1β、IL-6和TNF-α的表达显著升高(P<0.01);MTX组和EMS组IL-1β(除H-EMS组)、IL-6和TNF-α的表达显著低于CIA组(P<0.05).L-EMS组IL-1β、IL-6和TNF-α的表达显著低于M-EMS和H-EMS组(P<0.05,P<0.01).CIA组大鼠脾脏和关节中Gnαq mRNA和蛋白表达显著高于Control组(P<0.01);EMS和MTX组脾脏和关节中Gnαq mRNA和蛋白表达显著低于CIA组(P<0.05);1.5 g/kg EMS组Gnαq mRNA和蛋白的表达量明显低于其他组(P<0.01,P<0.05).不同实验组大鼠脾脏和关节中均检测到不同程度的阳性信号.结论:CIA大鼠关节和脾脏组织中 Gnαq表达增强可能导致炎症反应,可能通过下调Gnαq减轻CIA大鼠炎症反应.
Effects of Chinese herbal formula Er-miao-san on Gnαq expression and improvement of inflammatory reaction in CIA model rats
Objective:To explore the effect of different concentrations of Chinese herbal formula,Er-miao-san(EMS)immune regulatory gene on Gnαq expression in rats with collagen-induced arthritis(CIA).Methods:In the study,we established a CIA model,and randomly divided into model groups,EMS group with 1.5,3.0 and 4.5 g/kg(L-EMS,M-EMS,H-EMS)were adminis-tered to CIA rats by gavage,methotrexate was used as a positive control group(MTX).The rats in each group were given correspond-ing drugs for treatment,and the normal control group and model group were given the same amount of normal saline gavage.ELISA detects the expression of IL-1β,IL-6 and TNF-α in rats'serum.The mRNA and protein expression of Gnαq were analyzed using qRT-PCR and Western blot.The localization of Gnαq was performed by IHC.Results:The joint of rats in CIA group showed obvious damage,tissue hyperplasia and inflammatory infiltration;the joint tissue destruction and proliferation of MTX and EMS groups were reduced;the expressions of IL-1β,IL-6 and TNF-α in CIA group were extremely increased(P<0.01).The expressions of IL-1β(except H-EMS group),IL-6 and TNF-α in MTX and EMS groups were significantly lower than those in CIA group(P<0.05).The expressions of IL-1β,IL-6 and TNF-α in L-EMS group were lower than those in the M-EMS and H-EMS groups(P<0.05,P<0.01).The expression of Gnαq mRNA and protein in spleen and joints of CIA group were higher than those of Control group(P<0.01).The expression of Gnαq mRNA and protein in spleen and joints of EMS and MTX groups were lower than those of CIA group(P<0.05).The expression of Gnαq mRNA and protein in the dose of 1.5 g/kg of EMS group was lower than those of the other groups(P<0.01,P<0.05).Different degree of posi-tive signal was detected in spleen and joints in different groups.Conclusion:Gnαq is expressed in spleen and joints of CIA rats,may participate in the formation of inflammatory response in CIA rats,and then mediate the formation and development of RA by down-reg-ulating the expression of Gnαq.

Er-miao-sanGnαqRheumatoid arthritisSpleenJoints

芦文静、李振、郝慧琴、高玉亭、王泽、刘进

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山西中医药大学中西医结合基础实验室,晋中 030619

山西中医药大学基础医学院,晋中 030619

二妙散 Gnαq 类风湿关节炎 脾脏 关节

国家自然科学基金山西省自然科学基金面上项目山西中医药大学基础研究专项山西中医药大学基础研究专项山西中医药大学博士启动基金山西中医药大学青年科学家培育专项山西中医药大学科技创新团队重点项目山西省研究生教育创新项目山西中医药大学校级研究生创新项目

81904034201801D1212282020PY-JCY-072020PY-JCY-082020BK022021PY-QN-042018TD-0112019SY5162020CX023

2024

10.3969/j.issn.1000-484X.2024.06.018

中国免疫学杂志
中国免疫学会,吉林省医学期刊社

中国免疫学杂志

CSTPCD北大核心
影响因子：0.926
ISSN：1000-484X
年,卷(期)：2024.40(6)
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