IL-39在K562细胞中的作用及机制研究
Role and mechanism of IL-39 in K562 cells
吕康康 1徐明珠 1胡博 1刘跃均1
作者信息
- 1. 苏州大学附属第一医院,苏州 215006
- 折叠
摘要
目的:初步探索IL-39在K562细胞中的作用及机制.方法:qRT-PCR检测K562细胞IL-39R及STAT1/STAT3表达;Western blot检测K562细胞磷酸化的STAT1/STAT3及STAT1/STAT3蛋白水平;转录组学测序预测IL-39在K562细胞中调控的mRNA.结果:rIL-39显著促进K562细胞表面IL-39R的表达;IL-39在K562细胞中通过STAT1通路发挥作用;IL-39能够显著调控K562细胞中mRNA的表达,从而影响一系列生物学过程.结论:IL-39能够直接作用于K562细胞,显著改变K562细胞中的基因转录表达,继而通过STAT1通路发挥抗肿瘤效应,提示IL-39可能以相同方式作用于人白血病细胞.
Abstract
Objective:To explore the role and mechanism of IL-39 in K562 cells.Methods:The expressions of IL-39R and STAT1/STAT3 in K562 cells were detected via qRT-PCR;the protein levels of phospho-STAT1/STAT3 and STAT1/STAT3 in K562 cells were detected by Western blot;the mRNA regulated by IL-39 in K562 cells was predicted by RNA sequencing.Results:rIL-39 significantly promoted the expression of IL-39R in K562 cells;IL-39 affected K562 cells through STAT1 pathway;IL-39 significantly regulated the expression of mRNA in K562 cells,thus potentially affecting a series of biological processes.Conclusion:IL-39 can directly act on K562 cells,alter gene transcriptional expression and inhibit tumor growth through the STAT1 pathway.IL-39 may similarly modulate gene expression profile in human leukemia cells.
关键词
IL-12/IL-39/K562细胞/白血病Key words
IL-12/IL-39/K562 cells/Leukemia引用本文复制引用
基金项目
国家自然科学基金青年基金项目(81500146)
江苏省自然科学基金青年基金项目(BK20150356)
国家血液系统疾病临床医学研究中心转化研究课题(2020WSC05)
出版年
2024
国家科技期刊平台
NSTL
万方数据