全反式维甲酸对肝纤维化氧化应激损伤自然杀伤细胞的保护作用
Protective effect of all-trans retinoic acid on natural killer cells damaged by oxidative stress in liver fibrosis
平大冰 1齐婧姝 1孙鑫 1黄恺 1彭渊 1刘成海1
作者信息
- 1. 上海中医药大学附属曙光医院,上海 201203
- 折叠
摘要
目的:探讨全反式维甲酸(ATRA)对肝纤维化氧化应激损伤NK细胞功能的保护作用.方法:采用CCl4制备肝纤维化小鼠模型,通过生化及病理检测评价小鼠肝脏受损及纤维化程度,观察小鼠肝组织NK细胞变化及氧化应激损伤;体外采用H2O2诱导的NK92细胞株氧化应激损伤模型评价ATRA对NK92细胞的保护作用.结果:ATRA可以改善CCl4模型小鼠肝功能,抑制肝脏炎症及纤维化,增加肝脏SOD活性和GSH含量,促进肝内NK细胞比例和数量;体外实验证实ATRA可以保护H2O2诱导的NK细胞氧化损伤及凋亡.结论:ATRA可以改善CCl4引起的肝损伤及纤维化,可能与其保护NK细胞氧化应激损伤有关.
Abstract
Objective:To investigate the protective effect of all-trans retinoic acid(ATRA)on the function of NK cells dam-aged by oxidative stress in liver fibrosis.Methods:Mouse model with liver injury was established by carbon tetrachloride(CCl4),biochemical and pathological assays were used to evaluate the degree of liver injury and fibrosis in mice,and changes of NK cells and oxidative stress injury in liver tissue of mice were observed.In vitro,NK92 cell lines damaged by H2O2 oxidative stress were evaluated the protective effect of ATRA.Results:Liver inflammation and fibrosis were suppressed and liver function was improved in CCl4 model mice by ATRA treatment.ATRA could increase SOD activity and GSH content in liver tissue,which promoted the proportion and num-ber of hepatic NK cells.ATRA could protect NK cells from oxidative damage and apoptosis induced by H2O2 in vitro.Conclusion:ATRA can ameliorate liver injury and fibrosis induced by CCl4,which may be related to the inhibition of oxidative stress on NK cells.
关键词
全反式维甲酸/肝纤维化/氧化应激/自然杀伤细胞Key words
ATRA/Liver fibrosis/Oxidative stress/NK cells引用本文复制引用
基金项目
国家自然科学基金(81730109)
国家自然科学基金(81874363)
国家自然科学基金(82174057)
上海中医药大学预算内项目(2021LK081)
上海市科学技术委员会启明星计划扬帆专项(22YF1449300)
出版年
2024
国家科技期刊平台
NSTL
万方数据