首页|IL-6/JAK2/STAT3通路中葛花解酲汤对脾虚湿热型溃疡性结肠炎"炎-癌转化"的预防作用

IL-6/JAK2/STAT3通路中葛花解酲汤对脾虚湿热型溃疡性结肠炎"炎-癌转化"的预防作用

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
目的:探讨葛花解酲汤对脾虚湿热型溃疡性结肠炎"炎-癌转化"(UC-UCAC)小鼠结肠组织IL-6/JAK2/STAT3信号通路的影响.方法:80只SPF级C57BL/6雄性小鼠随机选出10只作为空白组,其余70只为造模组.造模组在建立脾虚湿热模型后随机分为模型组(第1、2、3周期)、葛花解酲汤高、中、低剂量组、美沙拉嗪组,10只/组,以氧化偶氮甲烷(AOM)/葡聚糖硫酸钠(DSS)继续建立UC-UCAC转化模型.各组给予相应药物治疗4周.观察小鼠一般状态;统计小鼠疾病活动指数(DAI)评分;HE染色观察小鼠结肠黏膜组织病理;Western blot、IHC和RT-qPCR检测小鼠结肠组织EGFR、IL-6、JAK2、STAT3、p-STAT3蛋白和基因表达.结果:与空白组相比,模型组(第3周期)小鼠一般状态较差,结肠黏膜组织出现癌变,DAI评分、各目标蛋白及基因表达显著升高(P<0.01);与模型组(第3周期)相比,各治疗组小鼠一般状态有所恢复,结肠组织病理不同程度改善,除葛花解酲汤低剂量组外,其他各治疗组各目标蛋白及基因表达显著下降(P<0.01).结论:葛花解酲汤可能通过抑制IL-6/JAK2/STAT3信号通路激活破坏肿瘤炎症微环境,修复受损结肠黏膜组织,延缓UC-UCAC进程,预防UCAC.
Preventive effect of Gehua Jiecheng Decoction in IL-6/JAK2/STAT3 pathway in"inflammation-cancer transformation"of ulcerative colitis with spleen deficiency and damp-heat
Objective:To investigate effect of Gehua Jiecheng Decoction on IL-6/JAK2/STAT3 signaling pathway in colon tissue of ulcerative colitis with"inflammatory to cancer transformation"(UC-UCAC)mice of spleen deficiency and damp-heat type.Methods:Ten from 80 SPF C57BL/6 male mice were randomly selected as blank group and other 70 mice were selected as model group.After establishment of spleen deficient damp-heat model,mice were randomly divided into model group(1st,2nd and 3rd cycle),Gehua Jiecheng Decoction high,medium and low doses groups and mesalazine group,with 10 pieces per group.UC-UCAC transformation model was further established with azo methane oxide solution(AOM)/sodium glucan sulfate(DSS).Each group was treated with corresponding drugs for 4 weeks.General state of mice was observed.Score of disease activity index(DAI)was calculated.HE staining was used to observe colonic mucosa pathology of mice.EGFR,IL-6,JAK2,STAT3 and p-STAT3 proteins and genes expressions in mice colon tissues were detected by Western blot,IHC and RT-qPCR,respectively.Results:Compared with blank group,mice in model group(3rd cycle)were generally in a worse state,colon mucosal tissue was cancerous,DAI score,target proteins and genes expressions were significantly increased(P<0.01).Compared with model group(3rd cycle),general state of mice in all treatment groups were restored and colonic tissues pathology were improved to some extent.Target proteins and genes expressions in other treatment groups were significantly decreased except for Gehua Jiecheng Decoction low-dose group(P<0.01).Conclusion:Gehua Jiecheng Decoction may destroy tumor inflammatory microenvironment,repair damaged colonic mucosa tissue,delay inflamma-tion-cancer transformation process and prevent UCAC by inhibiting activation of IL-6/JAK2/STAT3 signaling pathway.

Gehua Jiecheng DecoctionUlcerative colitisInflammation-cancer transformationSpleen deficiency and damp-heatIL-6/JAK2/STAT3 signaling pathway

李晓玲、吴玉泓、李海龙、殷银霞、舍雅莉、郝民琦、梁永林

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甘肃中医药大学,兰州 730000

深圳大学附属华南医院,深圳 518111

北京中医药大学深圳医院(龙岗),深圳 518116

南方医科大学中西医结合医院,广州 510000

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葛花解酲汤 溃疡性结肠炎 炎-癌转化 脾虚湿热 IL-6/JAK2/STAT3信号通路

国家自然科学基金2021年甘肃省高等学校产业支撑计划项目敦煌医学与转化教育部重点实验室2018年度开放基金项目2022年度中医学一级学科"岐黄英才"导师专项基金博导项目

818608102021CYZC-03DHYX18-172022-5

2024

10.3969/j.issn.1000-484X.2024.07.018

中国免疫学杂志
中国免疫学会,吉林省医学期刊社

中国免疫学杂志

CSTPCD北大核心
影响因子：0.926
ISSN：1000-484X
年,卷(期)：2024.40(7)
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