雷公藤红素苷对子痫前期大鼠肝肾细胞凋亡的影响及保护作用
Effect of celastrol on apoptosis of liver and kidney cells in preeclampsia rats and its protective effect
吴义军 1柴小利2
作者信息
- 1. 黄冈市中心医院产科,黄冈 438000
- 2. 湖北科技学院附属浠水县人民医院妇产科,黄冈 438200
- 折叠
摘要
目的:揭示雷公藤红素苷(Cel)对子痫前期(PE)大鼠肝肾细胞凋亡的影响及保护作用.方法:50只妊娠SD大鼠随机分为5组(n=10):对照组(Control)、PE组、PE+低剂量Cel组(PE+L-Cel)、PE+中剂量Cel组(PE+M-Cel)、PE+高剂量Cel组(PE+H-Cel),尾静脉注射脂多糖诱导PE大鼠模型,孕第14～20天,PE+L-Cel组、PE+M-Cel组和PE+H-Cel组大鼠每天分别腹腔注射5、10、20 mg/(kg·d)Cel,Control组和PE组注射等体积生理盐水.治疗结束后分别检测各组大鼠收缩压(SBP)和尿白蛋白.TUNEL染色检测肝、肾组织细胞凋亡.免疫组织化学染色检测肝、肾组织Bcl-2和Caspase-9表达.ELISA检测大鼠血清IFN-γ、IL-4、IL-17和IL-10水平.结果:与PE组相比,PE+L-Cel组、PE+M-Cel组和PE+H-Cel组SBP和尿白蛋白水平降低(P<0.05),肝、肾组织细胞凋亡率降低(P<0.05),肝、肾组织Caspase-9阳性表达明显降低,Bcl-2水平明显升高,血清IFN-γ和IL-17水平降低(P<0.05),IL-4和IL-10水平升高(P<0.05).结论:Cel可减轻PE大鼠症状并抑制肝肾细胞凋亡,可能通过纠正PE大鼠Th1/Th2及Th17/Treg细胞失衡发挥治疗作用.
Abstract
Objective:To reveal effect of celastrol(Cel)on apoptosis of liver and kidney cells in preeclampsia(PE)rats and its protective effect.Methods:Fifty pregnant SD rats were randomly divided into 5 groups(n=10):Control group,PE group,PE+low-dose Cel group(PE+L-Cel),PE+medium-dose Cel group(PE+M-Cel),PE+high-dose Cel group(PE+H-Cel),PE rat model was induced by injection of lipopolysaccharide through tail vein.Rats in PE+L-Cel group,PE+M-Cel group and PE+H-Cel group were intraperitoneally injected with 5,10 and 20 mg/(kg·d)Cel daily from 14～20 day of pregnancy.Control group and PE group were in-jected with equal volume of normal saline.Systolic blood pressure(SBP)and urine albumin of rats in each group were measured after treatment.Cell apoptosis of liver and kidney tissues were detected by TUNEL staining.Bcl-2 and Caspase-9 expressions in liver and kidney tissues were detected by immunohistochemical staining.ELISA was used to detect serum IFN-γ,IL-4,IL-17 and IL-10 levels.Results:Compared with PE group,SBP and urine albumin levels of PE+L-Cel group,PE+M-Cel group and PE+H-Cel group were decreased(P<0.05),cells apoptosis rates of liver and kidney tissues were decreased(P<0.05),positive expression of Caspase-9 was significantly reduced,Bcl-2 level was significantly increased,serum IFN-γ and IL-17 levels were reduced(P<0.05),while IL-4 and IL-10 levels were increased(P<0.05).Conclusion:Cel can reduce symptoms of PE rats and inhibit liver and kidney cell apoptosis,which may play a therapeutic effect by correcting imbalance of Th1/Th2 and Th17/Treg cells in PE rats.
关键词
雷公藤红素苷/子痫前期/细胞凋亡/T细胞亚群/炎症Key words
Celastrol/Preeclampsia/Apoptosis/T cell subsets/Inflammation引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据