Sevoflurane inhibits M1-type polarization of alveolar macrophages induced by lipopolysaccharide through p38/MAPK signaling pathway
Objective:To investigate the effect of sevoflurane on M1 polarization of alveolar macrophages induced by lipopoly-saccharide(LPS)by regulating p38/MAPK signaling pathway.Methods:RAW264.7 cells were stimulated with 1 μg/ml LPS for 24 h and exposed to different concentrations(1%,2%,4%)of sevoflurane.After that,MTT,ELISA,RT-qPCR and Western blot assays were used to detect cell viability,inflammation factors and signaling pathway-related protein levels.Results:LPS stimulation led to de-creased cell viability and M2-type anti-inflammatory cytokine levels,and increased M1-type pro-inflammatory cytokine levels and phosphorylation of p38/MAPK in RAW264.7 cells(P<0.05).After sevoflurane treatment,the cell viability and anti-inflammatory cyto-kine levels were significantly enhanced,and the pro-inflammatory cytokine levels and phosphorylation of p38 were significantly de-creased in a concentration-dependent manner(P<0.05).The p38/MAPK inhibitor SB202190 intensified the inhibitory effect of sevoflu-rane on M1-type polarization of alveolar macrophages.Conclusion:Sevoflurane inhibits LPS-induced M1 polarization of alveolar mac-rophages,possibly by regulation of the p38/MAPK signaling pathway.
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