Impact of Jiangzhi Zhuyu Decoction on lipid metabolism in atherosclerotic rats based on AMPK/PGC1α signaling pathway
Objective:To explore impact of Jiangzhi Zhuyu Decoction on lipid metabolism in atherosclerotic(AS)rats based on AMPK/PGC1α signaling pathway.Methods:AS model rats were induced and established by high-fat diet feeding and intraperitoneal injection of vitamin D3,and randomly grouped into model group,low-dose Jiangzhi Zhuyu Decoction(3.75 g/kg)group,medium-dose Jiangzhi Zhuyu Decoction(7.5 g/kg)group,high-dose Jiangzhi Zhuyu Decoction(15 g/kg)group,high-dose Jiangzhi Zhuyu Decoc-tion(15 g/kg)+doxorphine hydrochloride(AMPK inhibitor,0.2 mg/kg)group,with 12 rats in each group,another 12 normal SD rats were fed with normal diet and intraperitoneally injected with normal saline as control group.After Jiangzhi Zhuyu Decoction and doxor-phine hydrochloride were grouped to intervene rats in each group,levels of triacylglycerol(TG),total cholesterol(TC),high-density lipoprotein cholesterol(HDL-C)and low-density lipoprotein cholesterol(LDL-C)in rats were measured;HE staining was used to detect pathological changes of rat aorta;oil red O staining was used to detect lipid deposition in aorta and liver of rats in each group;microplate reader was used to detect levels of serum inflammatory mediators IL-8,IL-1β and IL-18;Western blot was used to detect expression of AMPK/PGC1α pathway protein in rat aorta.Results:Compared with control group,model group had severe pathological damage to aorta tissue,a large amount of lipid deposition in aorta and liver tissue,and severe liver steatosis,serum LDL-C,TC,TG,IL-8,IL-1β and IL-18 levels were obviously increased(P<0.05),serum HDL-C level,aortic tissue p-AMPK/AMPK and PGC1α protein expression were obviously decreased(P<0.05).Compared with model group,aortic tissue pathological damage,aortic lipid deposition and hepatic steatosis symptoms of rats in Jiangzhi Zhuyu Decoction groups were alleviated,serum LDL-C,TC,TG,IL-8,IL-1β and IL-18 were obviously decreased(P<0.05),serum HDL-C level,aortic tissue p-AMPK/AMPK and PGC1α protein expres-sions were obviously increased(P<0.05),which in a dose-dependent manner(P<0.05);compared with high-dose Jiangzhi Zhuyu Decoction group,aortic tissue pathological damage,aortic lipid deposition and liver steatosis were aggravated in high-dose Jiangzhi Zhuyu Decoction+doxorphine hydrochloride group,serum LDL-C,TC,TG,IL-8,IL-1β,and IL-18 levels were obviously increased(P<0.05),serum HDL-C level,aortic tissue p-AMPK/AMPK and PGC1α protein expressions were obviously decreased(P<0.05).Conclusion:Jiangzhi Zhuyu Decoction can resist occurrence and development of inflammation by activating AMPK/PGC1α signal,alleviate aortic pathological damage,aortic lipid deposition and liver steatosis symptoms in rats,and improve lipid metabolism in AS rats.
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