十二烷基硫酸钠诱导的小鼠刺激性接触性皮炎及局部免疫微环境分析

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国家科技期刊平台
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中文摘要：目的:建立十二烷基硫酸钠(SDS)诱发的小鼠刺激性接触性皮炎(ICD)模型，分析内在型，为精准治疗提供实验和理论依据。方法:小鼠随机分为两组(模型组和对照组)，局部涂抹4%SDS建立小鼠ICD模型，对照组涂抹生理盐水，剂量和频率与模型组保持一致，观察皮损情况。HE染色、甲苯胺蓝染色及免疫荧光染色分析表皮厚度及炎症细胞浸润程度，定量PCR分析细胞因子mRNA表达。结果:与对照组相比，ICD模型组小鼠颈背部皮肤表皮增厚、真皮层炎症细胞增多;中性粒细胞、巨噬细胞及T细胞增加;Il17a及Il17f mRNA表达上调。结论:成功建立SDS诱导的ICD模型，中性粒细胞、巨噬细胞及T细胞显著浸润，并伴有17型细胞因子分泌增加。

外文标题：Local immune microenvironment analysis of sodium dodecyl sulfate-induced irritant contact dermatitis model in mice

外文摘要：Objective:To establish an irritant contact dermatitis(ICD)model induced by sodium dodecyl sulfate(SDS)in mice,and explore its endotype to provide an experimental and theoretical basis for subsequent precise treatment.Methods:Mice were randomly divided into two groups(model group and control group),4%SDS was topically applied to induce ICD in mice,saline was used on control group,the dose and frequency were consistent with model group,and the skin lesions of mice were observed.Epidermal thickness and inflammatory cell infiltration were analyzed by HE staining,toluidine blue staining and immunofluorescence staining.Real-time quantitative PCR was performed to investigate mRNA expression levels of cytokines.Results:Compared with control group,mice in ICD model group showed epidermal thickness on the back of neck,and the numbers of inflammatory cells were increased in dermis.The number of neutrophils,macrophages and T cells were increased.Expressions of Il17a and Il17f mRNA levels were increased.Conclusion:SDS-induced ICD model is successfully established,with the elevated infiltration of neutrophils,macrophages and T cells,and secretion of type 17 cytokines.

外文关键词：

Irritant contact dermatitisEndotypeNeutrophilType 17 inflammationIL-17A

作者：

曾月玲、范立民、陈惠芳、何颖、陶爱林、刘雪婷

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作者单位：

广州医科大学附属第二医院,广东省过敏反应与免疫重点实验室,呼吸疾病国家重点实验室变态反应研究室,广州 510260

关键词：

刺激性接触性皮炎内在型中性粒细胞 17型炎症白细胞介素17A

基金：

国家自然科学基金国家自然科学基金广东省自然科学基金广州市科信局市校(院)联合资助(高水平大学)基础研究项目农业生物育种重大专项广州市教育局产学研科研项目

项目编号：

82371797821717642023A15150124842024A03J09422023ZD040630314CXY03

出版年：

2024

DOI：

10.3969/j.issn.1000-484X.2024.10.002

中国免疫学杂志

中国免疫学会,吉林省医学期刊社

中国免疫学杂志

CSTPCD北大核心

影响因子：0.926

ISSN：1000-484X

年,卷(期)：2024.40(10)