中国免疫学杂志2024，Vol.40Issue(10) ：2091-2094,2100.DOI:10.3969/j.issn.1000-484X.2024.10.012

非小细胞肺癌不同胸腔积液严重程度及预后患者lncRNA MEG3表达及其与Th17/CD4+T细胞的关系

lncRNA MEG3 expression and its relationship with Th17/CD4+T cells in non-small cell lung cancer patients with different severity and prognosis of pleural effusion

郭伟峰 何约明 庄锡彬 黄弘 真滢 朱秀妮 方耀堂 庄梓勋 曾玉叶
中国免疫学杂志2024，Vol.40Issue(10) ：2091-2094,2100.DOI:10.3969/j.issn.1000-484X.2024.10.012
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非小细胞肺癌不同胸腔积液严重程度及预后患者lncRNA MEG3表达及其与Th17/CD4+T细胞的关系

lncRNA MEG3 expression and its relationship with Th17/CD4+T cells in non-small cell lung cancer patients with different severity and prognosis of pleural effusion

郭伟峰 1何约明 1庄锡彬 1黄弘 1真滢 2朱秀妮 2方耀堂 2庄梓勋 2曾玉叶2
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作者信息

  • 1. 福建医科大学附属泉州第一医院呼吸与危重症医学科,泉州 362000
  • 2. 福建医科大学第三临床医学院,福州 350001
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摘要

目的:研究非小细胞肺癌(NSCLC)不同胸腔积液严重程度及预后患者lncRNA MEG3表达及其与Th17/CD4+T细胞的关系.方法:选取2020年1月至2022年12月福建医科大学附属泉州第一医院收治的104例NSCLC恶性胸腔积液患者作为研究对象,根据胸腔积液量分为3组:少量胸腔积液组(35例)、中量胸腔积液组(42例)、大量胸腔积液组(27例).根据患者疾病实际发展转归分为预后良好组(29例未出现复发和转移)和预后不良组(75例出现复发和转移).另选取同期于福建医科大学附属泉州第一医院治疗的60例肺炎良性胸腔积液患者作为对照组.实时荧光定量PCR检测两组胸腔积液中MEG3表达.收集受试者外周静脉血,流式细胞术检测外周血Th17细胞、CD4+T细胞比例,并计算Th17/CD4+T.对比各组患者lncRNA MEG3及外周血Th17、CD4+T细胞水平.Logistic回归分析NSCLC胸腔积液及预后的影响因素.结果:NSCLC组胸腔积液lncRNA MEG3表达及CD4+T细胞百分比低于对照组,Th17细胞百分比、Th17/CD4+T高于对照组(P<0.05).大量胸腔积液组lncRNA MEG3表达及CD4+T细胞百分比低于少量胸腔积液组、中量胸腔积液组,中量胸腔积液组lncRNA MEG3表达及CD4+T细胞百分比低于少量胸腔积液组,大量胸腔积液组Th17细胞百分比、Th17/CD4+T高于少量胸腔积液组、中量胸腔积液组,中量胸腔积液组Th17细胞百分比、Th17/CD4+T高于少量胸腔积液组(P<0.05).预后不良组lncRNA MEG3表达及CD4+T百分比低于预后良好组,而Th17细胞百分比、Th17/CD4+T高于预后良好组(P<0.05).Logistic回归分析结果显示,lncRNA MEG3为NSCLC胸腔积液的保护因素,Th17/CD4+T为危险因素(P<0.05);lncRNA MEG3为NSCLC预后的保护因素,Th17/CD4+T为危险因素(P<0.05).结论:NSCLC不同胸腔积液严重程度及预后患者lncRNA MEG3表达及Th17/CD4+T不同,且lncRNA MEG3为NSCLC胸腔积液及预后的保护因素,Th17/CD4+T为危险因素,可作为胸腔积液严重程度及预后诊断的有效生物标志物.

Abstract

Objective:To study lncRNA MEG3 expression and its relationship with Th17/CD4+T cells in patients with non-small cell lung cancer(NSCLC)with different pleural effusion severity and prognosis.Methods:A total of 104 NSCLC malignant pleural effusion patients admitted to Quanzhou First Hospital Affiliated to Fujian Medical University from January 2020 to December 2022 were selected as research subjects,and divided into three groups based on amount of pleural effusion,including small amount of pleural effusion group(35 cases),moderate amount of pleural effusion group(42 cases)and large amount of pleural effusion group(27 cases).According to actual development and prognosis of patient's disease,they were divided into good prognosis group(29 cases without recurrence and metastasis)and poor prognosis group(75 cases with recurrence and metastasis).Another 60 patients with benign pleural effusion due to pneumonia who were treated in Quanzhou First Hospital Affiliated to Fujian Medical University at same time were selected as control group.MEG3 expression in pleural effusion of two groups was detected by real-time fluorescent quantita-tive PCR,and peripheral venous blood of subjects was collected.Th17 cell and CD4+T cell ratios of peripheral blood were detected by flow cytometry,and Th17/CD4+T was calculated.lncRNA MEG3 and peripheral blood Th17 and CD4+T levels in each group of patients compared.Logistic regression analysis was used to analyze pleural effusion and prognostic factors in NSCLC.Results:lncRNA MEG3 expression and CD4+T percentage in pleural effusion in NSCLC group were lower than control group,while Th17 percentage and Th17/CD4+T were higher than control group(P<0.05).lncRNA MEG3 expression and CD4+T percentage in large pleural effusion group were lower than small and moderate pleural effusion groups.lncRNA MEG3 expression and CD4+T percentage in modarate pleural effusion group were lower than small pleural effusion group,while Th17 percentage and Th17/CD4+T in large pleural effusion group were higher than small and moderate pleural effusion groups.Th17/CD4+T was higher in small amount pleural effusion group(P<0.05).lncRNA MEG3 expression and CD4+T percentage in poor prognosis group were lower than those in good prognosis group,while Th17 percentage and Th17/CD4+T were higher than good prognosis group(P<0.05).Logistic regression analysis showed that lncRNA MEG3 was a protective factor for NSCLC pleural effusion,and Th17/CD4+T was a risk factor(P<0.05),lncRNA MEG3 was a protective factor of NSCLC prognosis,and Th17/CD4+T was a risk factor(P<0.05).Conclusion:lncRNA MEG3 expression and Th17/CD4+T in NSCLC patients with different pleural effusion severity and prognosis is not same.lncRNA MEG3 is a risk factor for NSCLC pleural effusion and prognosis,while Th17/CD4+T is a risk factor,which can be used as an effective biomarker for pleural effusion severity and progno-sis diagnosis.

关键词

非小细胞肺癌/胸腔积液/lncRNA/MEG3/Th17/CD4+T

Key words

Non-small cell lung cancer/Pleural effusion/lncRNA MEG3/Th17/CD4+T

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出版年

2024
中国免疫学杂志
中国免疫学会,吉林省医学期刊社

中国免疫学杂志

CSTPCD北大核心
影响因子：0.926
ISSN：1000-484X
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