红花多糖治疗雌二醇诱导的小鼠胸腺萎缩的药效作用研究
Rapeutical effect of safflower polysaccharide on thymic atrophy induced by estradiol in mice
蔡犇 1业康 2张巍 1姚佳丽 2解卓奇 1黄维 1卢敏1
作者信息
- 1. 浙江永宁药业股份有限公司,台州 318020
- 2. 浙江省中药研究所有限公司,杭州 310023
- 折叠
摘要
目的:雌二醇诱导小鼠胸腺萎缩模型,评价红花多糖治疗胸腺萎缩的药效.方法:75只雌性ICR小鼠分为5组:正常对照组、模型组、乌苯美司组、SPS高、低剂量组,除对照组外,其余各组隔天腹腔注射苯甲酸雌二醇注射液,共6次;药物治疗组于末次注射24 h后开始给药,1次/d,共10 d.末次给药24 h后牺牲小鼠,观察体质量、免疫器官指数、血浆MDA与GST水平、外周血细胞与T细胞变化,并对胸腺组织进行HE染色、TUNEL细胞凋亡染色,检测胸腺输出能力.结果:高、低剂量红花多糖均可显著提高小鼠胸腺指数(P<0.05),提高外周血白细胞数量(P<0.05)、CD3+CD4+T、CD3+CD8+T细胞比例(P<0.05)及CD4+T/CD8+T,并改善胸腺组织损伤.高剂量红花多糖可显著减少胸腺组织细胞凋亡,并提高胸腺输出能力.结论:红花多糖对雌二醇诱导的小鼠胸腺萎缩具有治疗作用.
Abstract
Objective:To evaluate efficacy of safflower polysaccharide in treatment of thymic atrophy induced by estradiol in mice.Methods:A total of 75 female ICR mice were divided into 5 groups:control group,model group,ubenimex group,SPS high dose group,SPS low dose group.Except for control group,each group was given intraperitoneal injection of estradiol benzoate every other day for 6 times.Treatment group began administration 24 h after the last parenteral administration,once a day for 10 days.Mice were sacrificed 24 hours after the last administration,and body weight,immune organ index,MDA and GST levels in plasma,periph-eral blood cells and T cells changes were observed,thymus tissue was stained by HE staining and TUNEL cell apoptosis staining,and thymus output capacity was detected.Results:Both high and low doses of safflower polysaccharide significantly improved thymus index(P<0.05)in mice,increased leukocytes level in peripheral blood(P<0.05),proportions of CD3+CD4+T,CD3+CD8+T cells(P<0.05)and CD4+T/CD8+T,improved thymus tissue damage.High dose of safflower polysaccharide could significantly reduce apoptosis in thymus tissue and enhance thymus output.Conclusion:Safflower polysaccharide has a certain therapeutic effect on estradiol-induced thymus atrophy in mice.
关键词
红花多糖/胸腺萎缩/雌二醇/治疗作用Key words
Safflower polysaccharide/Thymic atrophy/Estradiol/Therapeutical effect引用本文复制引用
基金项目
国家重点研发计划"中医药现代化"重点专项(2019YFC1710900)
出版年
2024
国家科技期刊平台
NSTL
万方数据