Immune Effect of PEDV Lactobacillus Engineering Strain in Mice
Porcine epidemic diarrhea virus(PEDV),which causes porcine epidemic diarrhea,was used as the research target in this study.The live vector strain LP1522-PEDS of Lactobacillus plantarum containing PEDV S gene was used to immunize mice orally.The content of SIgA in feces and the contents of IgG,IL-4,IL-2 and IFN-γ in serum of immunized mice were detected by ELISA,and the immune effect of Lactobacillus recombinant strain LP1522-PEDS on mice was evaluated.The results showed that after the first immunization,the contents of SIgA in feces and IgG,IL-4,IL-2 and IFN-γ in serum of the commercial inactivated vaccine group and the engineered strain immunization group were significantly higher than those of the control group(P<0.05).The antibody levels of the inactivated vaccine group and the recombinant strain group reached the maximum after 42-56 days of immunization.The levels of antibodies and immune factors were as follows:commercial inactivated vaccine group>LP1522-PEDS group>empty vector group ≈ control group.Subsequently,the antibody levels in the inactivated vaccine group and LP1522-PEDS group gradually decreased.After 70 days of immunization,the antibody level of the commercial inactivated vaccine group decreased with small amplitude and the antibody level of the engineered strain immunization group decreased greatly.The results showed that the genetic engineering Lactobacillus plantarum LP1522-PEDS carrying PEDV S gene could induce PEDV immune response in mice via oral administration.
万方数据
维普
