Preparation of Composite Emulsion Loaded with Milk Derived Antimicrobial Peptide BCp12
In this study,whey protein isolate(WPI)and carboxymethyl chitosan(CMCs)were used as carriers to load the milk derived antimicrobial peptide BCp12,and the antimicrobial rate of the composite lotion was used as an index to optimize the optimal preparation process.The potential crosslinking mechanism of the composite lotion was further revealed by scanning electron microscope(SEM)and molecular docking.The results showed that the best preparation process of the composite lotion was WPI:CMCs 1:1.5,the concentration of bcp12 was 1 mg/mL,and the antimicrobial rates against Escherichia coli and Staphylococcus aureus were 74.56±0.61%and 70.58±0.44%,respectively;The bacteriostatic diameters of Escherichia coli and Staphylococcus aureus were 20.50±1.08 mm and 19.33±0.85 mm,respectively.It can be seen from the scanning electron microscope that the composite lotion has fine and uniform texture,and the three substances have good compatibility.Molecular docking showed that the BCp12-WPI-CMCs composite lotion was mainly bound by hydrogen bonds formed by C = O,-NH2 and-OH,and the Y1 amino acid in BCp12 may be the best binding site.This study provided technical references for the application and development of BCp12-WPI-CMCs composite lotion.
Antimicrobial peptide BCp12Carboxymethyl chitosanWhey protein isolateScanning electron microscopeMolecular docking
NETL
NSTL
万方数据
维普
