Detection of Three SNPs and Their Association with Mastitis and Milk Production Traits in Chinese Holstein Cows
The purpose of this experiment was to screen and verify the functional genes with high mastitis resistance in Chinese Holstein cows population.The gene frequencies of three mastitis related gene loci in Beijing were analyzed using sequenom technology.At the same time,the gene polymorphism and its correlation with Somatic Cell Count(SCC)and milk production traits were analyzed.In this study,the polymorphisms of DCK and HIST1H2BK genes in 568 Chinese Holstein cows in Beijing were detected,and the relationship of different genotypes of three SNPs with SCC and milk production traits was analized.The results showed that SNP 6:g.86337334 A>G of the DCK gene was highly significantly correlated with the number of SCC(P<0.01),and the SNP 6:g.86322040 C>T[rs43472176]was also highly significantly correlated with SCC(P<0.01).The SNP g.31354269 T>G[rs41654340]was significantly associated with SCC(P<0.05),and was extremely significantly associated with the rate of milk fat and milk protein(P<0.01).The results showed that DCK and HIST1H2BK genes could be used as candidate genes for marker assisted selection affecting mastitis traits in Chinese Holstein cows,and the above SNPs were significantly correlated with SCC traits.It may directly or indirectly affect the milk fat or protein content traits of cows.This study lays a solid foundation for subsequent marker assisted selection in Holstein cows.
万方数据
维普
