Isolation,Identification and Drug Resistance Analysis of Mastitis Pathogenic Bacteria froma Large-scale Dairy Farm in Hunan Province
To ascertain the pathogenic species and drug resistance of clinical dairy mastitis in a large-scale dairy farm in Hunan,32 clinical-type dairy mastitis samples were subjected to traditional bacterial culture,biochemical tests,16S rDNA nucleotide sequence detection and drug sensitivity tests.The results showed that a total of 23 strains of E.coli,12 strains of Streptococcus udder and 5 strains of Klebsiella pneumoniae were isolated and identified without duplication,and the detection rates of the samples were 71.9%(23/32),37.5%(12/32)and 15.6%(5/32),respectively.The drug sensitivity tests showed that most of the E.coli and Streptococcus udder were resistant to penicillin,amoxicillin,ceftazidime,gentamicin and lincomycin serious,resistance rate of 50%～86.9%,to imipenem,tetracycline and erythromycin more sensitive,sensitivity rate of 75%～82.6%.The Klebsiella pneumoniae to most antibiotics bacteria resistance degree is low,to imipenem,amikacin,tetracycline,erythromycin sensitivity rate of 100%.It is suggested that the farm should grasp environmental hygiene,improve biosecurity,and establish a scientific and reasonable treatment plan.
Mastitis in dairy cowsIsolation and identification of bacteriaDrug sensitive test
万方数据
维普
