Changes in Immune Antibodies in Calves Vaccinated with Brucella A19-△VirB12 Vaccine
To understand the antibody changes in calves immunized with different doses of Brucella A19-△VirB12 vaccine using different vaccination methods,three to six month old female calves were immunized with different doses of Brucella A19-△VirB12 vaccine through eye contact and subcutaneous inoculation.Antibody changes in the serum collected from calves during different immunization periods were detected using in vitro agglutination test(SAT).In the first trial,SAT positive results were detected in all immune groups except for the 1 billion eye drops group and the 5 billion subcutaneous vaccination group after 24 weeks of immunization.In the second experiment,the SAT test positive rate of the 5 billion subcutaneous immunization group reached 84.21%after 3 weeks of immunization,and all SAT tests were negative after 25 weeks of immunization;The two eye spot immunization groups initially detected continuous antibody positivity after 12 weeks of immunization,and the antibody positivity rate was 4.54%～9.09%by 52 weeks of immunization.After high-dose immunization of calves with Brucella A19-△VirB12 vaccine,it may interfere with the determination of later brucellosis quarantine results.After subcutaneous immunization of calves with 5 billion CFU/mL of A19-△VirB12 vaccine,it has the advantages of good immunogenicity and early antibody conversion time.The antibody detection time of the eye contact immune group is late,and there is currently a lack of detection methods for the cellular immune indicators of the vaccine in the early stage,which requires further research.
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