Analysis of the Virulence and Drug Resistance of a Strain of Staphylococcus aureus Isolated from Raw Milk
In order to understand the characteristics of Staphylococcus aureus in raw milk,the S.aureus was isolated and identified in mixed fresh milk with conventional isolation methods combined with specific nuc gene PCR detection from a large-scale cattle farm in Xinjiang.The drug resistance phenotype of isolate to 11 antibiotics in 7 types of antibiotics were detected with K-B method,8 important virulence genes and 6 drug resistance genes of S.aureus were analyzed by PCR.The results showed that 1 S.aureus was isolated from raw milk samples,it was resistant to 3 antibiotics including penicillin,ampicillin et al,and was intermediate to 4 antibiotics,it carried sec,sea,hla,tsst-1,pvl,clfA 6 important virulence genes and qacA,msrA,ermC,mecA 4 drug resistance genes.The isolate caused obvious histopathological changes in infected mice and lead to death.The results indicated that the S.aureus isolated from raw milk had strong pathogenicity,carried many virulence genes and drug resistance genes and had extensive multidrug resistance.
Raw milkStaphylococcus aureusVirulence geneDrug resistance phenotypeDrug resistance gene
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万方数据
