一株分离于生鲜乳的金黄色葡萄球菌毒力与耐药性分析
Analysis of the Virulence and Drug Resistance of a Strain of Staphylococcus aureus Isolated from Raw Milk
王巧梅 1王子杰 1操义恒 1周霞 1吴洁 2王晓兰 1孙志华 1王震 1张辉1
作者信息
- 1. 石河子大学动物科技学院,石河子 832003
- 2. 新疆生产建设兵团第八师畜牧兽医工作站,石河子 832003
- 折叠
摘要
为了解生鲜乳中金黄色葡萄球菌的特性,试验通过无菌采集新疆某规模化牛场混合新鲜乳样,采用常规分离方法结合PCR方法检测特异性nuc基因分离鉴定金黄色葡萄球菌,分析分离菌株对7类11种抗生素的耐药表型,采用PCR方法对金黄色葡萄球菌常见重要的8种毒力基因和6种耐药基因进行检测并分析其致病性.结果表明新鲜乳样中分离到一株金黄色葡萄球菌;该菌对青霉素、氨苄西林耐药,对卡那霉素、四环素4种抗生素处于中介,对庆大霉素、红霉素、头孢唑林等5种抗生素敏感;携带sec、sea、hla、tsst-1、pvl、clfA 6种毒力基因和qacA、msrA、ermC、mecA 4种耐药基因;能引起感染小鼠明显的组织病理变化并导致死亡.说明分离于生鲜乳中的金黄色葡萄球菌携带众多毒力基因和耐药基因,具有广泛的多重耐药性.
Abstract
In order to understand the characteristics of Staphylococcus aureus in raw milk,the S.aureus was isolated and identified in mixed fresh milk with conventional isolation methods combined with specific nuc gene PCR detection from a large-scale cattle farm in Xinjiang.The drug resistance phenotype of isolate to 11 antibiotics in 7 types of antibiotics were detected with K-B method,8 important virulence genes and 6 drug resistance genes of S.aureus were analyzed by PCR.The results showed that 1 S.aureus was isolated from raw milk samples,it was resistant to 3 antibiotics including penicillin,ampicillin et al,and was intermediate to 4 antibiotics,it carried sec,sea,hla,tsst-1,pvl,clfA 6 important virulence genes and qacA,msrA,ermC,mecA 4 drug resistance genes.The isolate caused obvious histopathological changes in infected mice and lead to death.The results indicated that the S.aureus isolated from raw milk had strong pathogenicity,carried many virulence genes and drug resistance genes and had extensive multidrug resistance.
关键词
生鲜乳/金黄色葡萄球菌/毒力基因/耐药表型/耐药基因Key words
Raw milk/Staphylococcus aureus/Virulence gene/Drug resistance phenotype/Drug resistance gene引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据