摘要
弗荷杂交为乳肉牛融合发展的主要途径之一.为揭示弗荷杂交牛与荷斯坦牛在抗病力免疫细胞因子方面的差异,采用双抗体夹心酶联免疫吸附试验,检测弗荷杂交牛、荷斯坦牛与抗病力相关的主要免疫细胞因子白介素、补体、干扰素、免疫球蛋白、主要组织相容性复合体在血液中的浓度,为弗荷杂交牛抗病力研究提供理论支撑.经检测血清中免疫球蛋白IgM含量弗荷杂交牛显著高于荷斯坦牛(P<0.05),γ-干扰素、主要组织相容性复合体的含量弗荷杂交牛极显著高于荷斯坦牛(P<0.01),白介素IL-2、补体蛋白C3、C4、血清总补体CH50、干扰素γ、免疫球蛋白IgA含量弗荷杂交牛极显著低于荷斯坦牛(P<0.01),白介素IL-3、IL-6、IL-10、免疫球蛋白IgG含量弗荷杂交牛与荷斯坦牛差异不显著(P>0.05).本研究从免疫细胞因子的层面揭示了两种牛存在的差异,可为弗荷杂交的抗病育种提供参考.
Abstract
As one of the main ways of fusion development of dairy cattle,a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) was used to reveal the differences of immune cytokines in disease resistance between Fer-Hos and Holstein cattle.The blood concentrations of interleukin,complement,interferon,immunoglobulin and major histocompatibility complex related to disease resistance of Feuho hybrid cattle and Holstein cattle were detected to provide theoretical support for the study of disease resistance of Feuho hybrid cattle.The serum immunoglobulin IgM content of Verho hybrid cattle was significantly higher than that of Holstein cattle (P<0.05),and the serum gamma-interferon and major histocompatibility complex(MHC) contents of Verho hybrid cattle were significantly higher than that of Holstein cattle (P<0.01).The contents of interleukin IL-2,complement protein C3,C4,serum total complement CH50,interferon γ and immunoglobulin IgA were significantly lower than those of Holstein cattle (P<0.01),but there were no significant differences in the contents of interleukin IL-3,IL-6,IL-10 and immunoglobulin IgG between Feuger hybrid cattle and Holstein cattle (P>0.05).The differences between the two breeds of cattle were revealed from the level of immune cytokines,which provided reference for the disease resistance breeding of Franco-Dutch crossing.
维普
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