A Study on Concentration of Various Cytokines in Friesian Crossbred Cattle and Holstein Cattle
As one of the main ways of fusion development of dairy cattle,a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) was used to reveal the differences of immune cytokines in disease resistance between Fer-Hos and Holstein cattle.The blood concentrations of interleukin,complement,interferon,immunoglobulin and major histocompatibility complex related to disease resistance of Feuho hybrid cattle and Holstein cattle were detected to provide theoretical support for the study of disease resistance of Feuho hybrid cattle.The serum immunoglobulin IgM content of Verho hybrid cattle was significantly higher than that of Holstein cattle (P<0.05),and the serum gamma-interferon and major histocompatibility complex(MHC) contents of Verho hybrid cattle were significantly higher than that of Holstein cattle (P<0.01).The contents of interleukin IL-2,complement protein C3,C4,serum total complement CH50,interferon γ and immunoglobulin IgA were significantly lower than those of Holstein cattle (P<0.01),but there were no significant differences in the contents of interleukin IL-3,IL-6,IL-10 and immunoglobulin IgG between Feuger hybrid cattle and Holstein cattle (P>0.05).The differences between the two breeds of cattle were revealed from the level of immune cytokines,which provided reference for the disease resistance breeding of Franco-Dutch crossing.