中国奶牛2024,Issue(12) :25-28.DOI:10.19305/j.cnki.11-3009/s.2024.12.006

新疆石河子规模化牛场牛支原体肺炎流行病学调查

Epidemiological Investigation of Bovine Mycoplasma Pneumonia in Large-Scale Cattle Farms in Shihezi,Xinjiang

胡南方 叶翠芳 赵宗胜 张红梅 李广 郑炜 林为民
中国奶牛2024,Issue(12) :25-28.DOI:10.19305/j.cnki.11-3009/s.2024.12.006

新疆石河子规模化牛场牛支原体肺炎流行病学调查

Epidemiological Investigation of Bovine Mycoplasma Pneumonia in Large-Scale Cattle Farms in Shihezi,Xinjiang

胡南方 1叶翠芳 1赵宗胜 1张红梅 2李广 3郑炜 1林为民3
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作者信息

  • 1. 石河子大学动物科技学院,石河子 832000
  • 2. 石河子大学第一附属医院,石河子 832000
  • 3. 新疆兵团第八师畜牧兽医工作站,石河子 832000
  • 折叠

摘要

为初步了解石河子地区规模化牛场2023年牛支原体肺炎流行情况,对该地区5个不同规模牛场血清样本进行牛支原体血清抗体及牛甘露聚糖结合凝集素2(MBL2)水平检测,分析血清学调查结果.同时,采集该地区20头有呼吸道症状的牛鼻腔黏液,采用PCR检测其病原体.结果显示,1~5月龄牛支原体血清抗体阳性率平均为38.95%,1、3、4、5月龄犊牛抗体阳性率分别为40%、34.25%、46.67%、53.85%,MBL2检测结果显示超过正常表达量的牛只数量占比为37.13%,2、3月龄犊牛MBL2含量超过正常表达的牛只数量占比分别为50%、46.57%.70%(14/20)的牛鼻腔黏液样本中检测出相关病原体存在,其中3月龄犊牛死亡率最高为27.27%.结果表明,石河子地区部分规模化牛场牛支原体病流行.

Abstract

In order to have a preliminary understanding of the prevalence of Mycoplasma bovis pneumonia in large-scale cattle farms in the Shihezi region in 2023,serum samples from five different-sized cattle farms in the region were tested for Mycoplasma bovis serum antibodies and bovine mannan-binding lectin 2 (MBL2) levels,and the serological findings were analyzed.Meanwhile,nasal mucus was collected from 20 cattle with respiratory symptoms in the region,and PCR was used to detect the pathogen.The results showed that the average Mycoplasma bovis antibody positivity rate was 38.95% at 1-5 months.The antibody positivity rate was 40%,34.25%,46.67%,and 53.85% in calves at 1,3,4,and 5 months,respectively.The results of the MBL2 assay showed that the percentage of MBL2 level exceeding the regular expression was 37.13%,and the percentage of MBL2 level exceeding the regular expression in calves at the age of 2 and 3 months was 50%,respectively,46.57%.70% (14/20) of the nasal mucus samples from cows were detected with related pathogens,and the mortality rate of 3-month-old calves was the highest at 27.27%.The results indicated that bovine mycoplasmosis was prevalent in some large-scale cattle farms in the Shihezi area.

关键词

牛支原体肺炎/PCR/ELISA/MBL2/流行病学调查

Key words

Mycoplasma bovine pneumonia/PCR/ELISA/MBL2/Epidemiological investigation

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出版年

2024
中国奶牛
中国奶业协会

中国奶牛

影响因子:0.416
ISSN:1004-4264
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