首页|采用高通量测序技术对生驼乳中细菌群落多样性研究

采用高通量测序技术对生驼乳中细菌群落多样性研究

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为全面了解生驼乳中的细菌多样性,采用Illumina Miseq高通量测序技术分析生驼乳中的菌落结构和优势菌群.对四个季度从乌鲁木齐市及木垒县采集的96个驼乳样本,提取全基因组,通过高通量测序方法进行研究,获得多样性信息.结果显示,96个样本测序共获得7254239对有效序列,夏季特定OTU数量高达84个,春季特定OTU数量较多,冬季相对较低.本研究在高通量测序技术手段的分析下,驼乳的细菌群落共分为34个门、87个纲、131个目、254个科、587个属、412个种.驼乳的细菌群落从门水平上共分离出34种,优势菌群为变形菌门(Proteobacteria).影响驼乳样本中微生物群落结构组成差异的主要因素是季节.
Study of Bacterial Community Diversity in Raw Camel Milk by High-throughput Sequencing Technology
To get a comprehensive understanding of bacterial diversity in raw camel milk,Illumina Miseq high-throughput sequencing technology was used to analyse the colony structure and dominant flora in raw camel milk.For 96 camel milk samples collected from Urumqi City and Mubi County in four seasons,the whole genome was extracted and studied by high-throughput sequencing methods to obtain diversity information.A total of 7,254,239 valid sequence pairs were obtained from the sequencing of 96 samples,and the number of specific OTUs was as high as 84 in summer,higher in spring and relatively low in winter.In this study,the bacterial community of camel's milk was classified into 34 phyla,87 classes,131 orders,254 families,587 genera,and 412 species when analysed by high-throughput sequencing technological tools.A total of 34 species were isolated from the bacterial community of camel's milk at the phylum level,and the dominant colony was Proteobacteria.The main factor affecting the differences in the structural composition of microbial communities in camel's milk samples was season.

High-throughput sequencingRaw camel milkBacterial diversity

王帅、王富兰、赵艳坤、武亚婷、李新霞、马宪兰、陈贺

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新疆农业科学院农业质量标准与检测技术研究所/农业部农产品质量安全风险评估实验室/新疆 农产品质量安全实验室,乌鲁木齐 830091

高通量测序 生驼乳 细菌多样性

2024

中国奶牛
中国奶业协会

中国奶牛

影响因子:0.416
ISSN:1004-4264
年,卷(期):2024.(12)