Study of Bacterial Community Diversity in Raw Camel Milk by High-throughput Sequencing Technology
To get a comprehensive understanding of bacterial diversity in raw camel milk,Illumina Miseq high-throughput sequencing technology was used to analyse the colony structure and dominant flora in raw camel milk.For 96 camel milk samples collected from Urumqi City and Mubi County in four seasons,the whole genome was extracted and studied by high-throughput sequencing methods to obtain diversity information.A total of 7,254,239 valid sequence pairs were obtained from the sequencing of 96 samples,and the number of specific OTUs was as high as 84 in summer,higher in spring and relatively low in winter.In this study,the bacterial community of camel's milk was classified into 34 phyla,87 classes,131 orders,254 families,587 genera,and 412 species when analysed by high-throughput sequencing technological tools.A total of 34 species were isolated from the bacterial community of camel's milk at the phylum level,and the dominant colony was Proteobacteria.The main factor affecting the differences in the structural composition of microbial communities in camel's milk samples was season.