Cloning & Expression Analysis of WRKY Gene Segment in Brassica campestris
The WRKY transcription factor is considered to be involved in biotic and abiotic stress responses widely in plants. The purpose of the paper is to study the relative expression trends of WPKY transcription factor gene under ABA treatment in Brassica campestris. Actin gene and WRKY gene were cloned by using the homology cloning method. The sequences of nucleic acid and amino acid were analyzed using BLAST and DNAMAN software. Relative expression trends of WRKY gene were detected by applying real-time relative quantification PCR (RT-qPCR) under ABA (100 μmol/L) treatment; A 680 bp WRKY gene segment and a 933 bp Actin gene segment were acquired in Brassica campestris. The result of RT-qPCR analysis revealed that the relative expression of WRKY gene reached the peak at 1 h with ABA treatment. In this paper, Actin and WRKY gene in Brassica campestris were cloned successfully, which was proved to play an important role in ABA signal pathway.
NETL
NSTL
万方数据
维普
