甘薯叶片和叶柄组织诱导培养及植株再生研究
Study of Leaf and Petiole Tissue Culture Induction and Plant Regeneration on Sweet Potato
徐茜 1乐正碧 1徐燕 1文明玲 1王良平 1余鸿燕 1黎华 1张菡1
作者信息
- 1. 重庆三峡农业科学院,重庆万州404155
- 折叠
摘要
为探索甘薯叶片和叶柄组织诱导和植株再生技术,应用甘薯优良品种‘万薯7号'叶片、叶柄在7个诱导培养基中的离体培养.试验表明,‘万薯7号'叶片和叶柄在本试验的7个诱导培养基中,极易诱导产生愈伤组织,诱导率达100%.叶片和叶柄在经培养基MS+2.0 mg/LKT+0.5.mg/L IAA诱导出绿色愈伤组织后,在继代培养基MS+4.0mg/L 6-BA+0.01 mg/LNAA培养15天后,均诱导成苗.
Abstract
It was studied the leaf and petiole tissue culture induction and plant regeneration with the sweet potato variety ‘ Wanshu No. 7’. The result indicated the sweet potato variety of leave and petiole could be easy to induce callus in 7 induction mediums, the induction rate of 100％. The leaf and petiole induced green callus in the medium of MS +2.0 mg/L KT +0.5 mg/L IAA, and then subculture 15 days in the medium of MS + 4.0 mg/L 6-BA + 0.01 mg/L NAA, they were induced emergence seeding.
关键词
甘薯/愈伤组织/植株再生Key words
sweet potato/callus/plant regeneration引用本文复制引用
基金项目
国家农业科技成果转化资金(2008GB2F100262)
重庆市攻关项目(CSTC)
重庆市攻关项目(2009AC1102)
重庆市甘薯工程技术研究中心(CSTC)
重庆市甘薯工程技术研究中心(2009CB1001)
出版年
2011
国家科技期刊平台
NSTL
维普
万方数据