Optimization of SRAP-PCR System and Primers Screening in Hydrangea
In order to make clear the optimizing SRAP-PCR reaction system of Hydrangea, 19 Hydrangea plants were used as material, the single factor experiment was designed in 11 levels of 5 factors (concentration of primer, Mg2 +, DNA template and dNTPs, and Taq DNA polymerase contents) respectively. The results showed that the reaction system obtained as followed: 2.5 μL 10×PCR Buffer, 30 ng DNA , 1.6 mmol/L MgCb 0.6 mmol/L dNTPs, 3.5 U Taq DNA polymerase, and in a total volume of 25 μL. This optimizing reaction system is suit for the SRAP genetic variation research of Hydrangea.
NETL
NSTL
万方数据
维普
