首页|小麦TaSnRK2.9蛋白激酶基因克隆与生物信息学分析

小麦TaSnRK2.9蛋白激酶基因克隆与生物信息学分析

扫码查看
原文链接
  • NETL
  • NSTL
  • 万方数据
  • 维普
为了揭示小麦TaSnRK2.9基因的结构特点和功能特性,为该基因的研究和应用提供基础,根据已报道的水稻蛋白激酶SAPK9基因(GenBank登录号AB125310.1)序列信息,利用RT-PCR技术克隆了小麦TaSnRK2.9基因,并对其进行生物信息学分析.结果表明:小麦TaSnRK2.9阅读框为1092bp,编码363个氨基酸,与水稻SAPK9亲缘关系最近,属于第三亚家族；亚细胞定位预测TaSnRK2.9定位在细胞质中；基因表达谱分析发现TaSnRK2.9在根、茎、叶、花等器官和组织中都有表达,并且在叶中的表达量最高.TaSnRK2.9的克隆为研究其在小麦中的功能提供基本信息和指导.
Identification and Bioinformatics Analysis of TaSnRK2.9in Wheat
In order to reveal the structure and function characteristics of TaSnRK2.9 in wheat, and provide the basis for the research and application of this gene, the cDNA of TaSnRK2.9 was cloned in wheat by RT-PCR based on the nucleotide sequence of SAPK9 gene in rice, and the bioinformatics analysis was also finished. The results showed that the length of ORF was 1092 bp, encoding 363 amino acids. Sequence analysis and the phylogenetic trees showed that this gene was closely related to SAPK9 (AB125310) of rice, belonged to the subfamily III. Subcellular localization predicted TaSnRK2.9 gene located in the cytoplasm. Gene expression profile analysis found that TaSnRK2.9 gene expressed in roots, stems, leaf, flowers, and was more highly expressed in leaf. The cloning of the TaSnRK2.9 gene full length cDNA provided the basis and guidance for its characteristics and function.

wheatTaSnRK2.9\ cDNART-PCRphylogenetic analysisexpression pattern

连魏卫、唐益苗、高世庆、张朝、赵昌平

展开 >

首都师范大学生命科学学院,北京100048

北京杂交小麦工程技术研究中心,北京100097

小麦 TaSnRK2.9 cDNA RT-PCR 系统进化分析 表达谱

国家转基因生物新品种培育重大专项国家转基因生物新品种培育重大专项国家转基因生物新品种培育重大专项北京市科技新星项目北京市科技新星项目北京农林科学院院青年北京市自然科学基金

2008ZX08002-0022008ZX08002-0032008ZX08002-0042007B30562008B0355102016

2011

中国农学通报
中国农学会

中国农学通报

CSTPCDCSCD
影响因子：0.891
ISSN：1000-6850
年,卷(期)：2011.27(33)
  • 7
  • 31