The Over-expression Vector Construction of BnaiMyAP9 and BnaiMyAP12and Transformation of Arabidopsis thaliana
Inducible Myrosinase-associated protein (iMyAP) usually occur in complexes with Myrosinases and Myrosinases-binding protein plays important roles in the plant' s defense system. To study the functions of iMyAP in abiotic stress responses, we constructed two plant over-expression vectors CaMV35S::iMyAP9 and CaMV35S::iMyAP12. Genetic transformation of Arabidopsis was performed by floral dip method. The T0 seeds were screened on medium with kanamycin and the anti-kanamycin plants were tested by PCR to confirm if anti-Kan plants were transgenic plants. 67 transgenic plants were obtained. The results of RT-PCR showed that iMyAP9 and iMyAP12 genes efficiently expressed in the transgenic arabidopsis plants, which the results lay a foundation for further studying the biological function of iMyAP9 and iMyAP12 genes.
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