Genetic Transformation of Cassava with Its SBE Ⅰ Antisense Gene
This study aimed to gain transgenic 'SC-8' cassava with the transformation of starch branching enzyme (SBE I) antisense gene, and provide basis for using transgenic technology in the improvement of cassava starch quality. Based on the cassava regeneration system which could take the embryoid cotyledon to complete plants, the SBE I antisense gene was transformed by Agrobacterium-mediated transformation and the tuber-specific promoter Sporamin was used. 517 'SC-8' cassava cotyledons were inoculated which resulted in 7 well grown regenerated plants with a transformation rate of 1.35%. By PCR analysis, the SBE I antisense gene products were amplified in 5 regenerated plants which could provide evidence for the combination of SBE I antisense gene in cassava genome. The SBE I antisense gene can be successfully transformed to 'SC-8' cassava by the Agrobacterium-mediaied transformation method. 5 transgenic 'SC-8' cassava plants were obtained.
NETL
NSTL
万方数据
维普
